how to purify tau protein in low temperature by tris buffer?
How i clean PD minitrap G10 column???
27 November 2023 3,077 0 View
AND there Is a gold technique that Is suitible for all meassurment off nano world????
22 December 2022 8,393 3 View
Hello, Has anyone studied gene expression using the qMAXSenTM One-Step Green RT-qPCR Kit (High RoxTM), and if so, in which Real-Time PCR instrument?
12 May 2022 5,397 0 View
Increasing the capacity of the thermal energy storage system beyond 20 hours seems to pose an economic problem, as the cost of the tanks increases for higher capacities as a result of the...
19 January 2022 2,471 8 View
Hello, Is it possible to measure arginase activity in organs that have been stored at -60°C for 3 years?
27 September 2021 6,463 3 View
Hello everyone, I'm facing a problem when it comes to defining the parameters of Boussinesq approximation in Ansys Fluent. I'm simulating a fluid and a PCM with Boussinesq approximation, which...
18 September 2021 6,677 4 View
hello everyone The strains of parasites Leishmania wich I'm currently working with exceeded 10 passages. I would like to know if there is another way to recover their virulence, a part from...
03 April 2020 6,339 2 View
Hello everybody! I have an anti-IL17R neutralizing antibody, the stock concentration is 0.5mg/ml , what could be the used concentration to neutralize IL17 in cells culture?
07 January 2018 5,975 3 View
Hi everyone, I am currently working on the analysis of T lymphocyte sub populations in breast cancer tissues using flow cytometry, currently I have frozen breast tissues in DMSO 10 % SVF,...
13 November 2017 3,540 1 View
the activity of SO4 in sediment
29 June 2017 4,671 2 View
I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...
07 August 2024 505 3 View
Does anyone tried to do nucleofection with AMAXA by Lonza with lower than recommended amount of buffer in the cuvettes (100 ul)?
07 August 2024 4,616 0 View
Currently, when I run SDS-PAGE, I don't see any bands at all, even though I used the same material just a day ago and it worked fine.... In our lab, we dilute the 10X running buffer to 1X and...
06 August 2024 5,373 2 View
I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...
06 August 2024 1,367 3 View
During low-temperature testing, new diffraction peaks that appear could be indicative of several phenomena. In one of our tests, we observed notable new peaks around 40° and 45° in a specific...
06 August 2024 726 3 View
Hello everyone, I'm encountering an issue with my electrochemical impedance spectroscopy (EIS) measurements and would appreciate some insights. Experimental Setup: Electrodes: Gold interdigitated...
05 August 2024 3,783 2 View
Is it the "elution buffer" or the "dialysis buffer"? Note: I'll be using NanoDrop OneC
01 August 2024 967 3 View
A typical reforming section in a large-scale Haber-Bosch process (Figure 1) comprises two reactors. A mixture of methane (CH4) and steam (H2O) is fed into a primary reformer to produce syngas via...
01 August 2024 7,051 5 View
Hello everyone! How can i prepare EDTA buffer solution (pH 8.2) to determine GSH levels? Thank you in advance.
29 July 2024 4,374 1 View
I want to preserve the sample leaves for the LM study of the stomata and conidia analysis. Whether 2.5% glutaraldehyde in 0.05 M phosphate buffer with pH 7 can be applied for preservation.
29 July 2024 8,560 0 View
