Do you know any place or website to get Lymantria dispar eggs?

I need Lymantria dispar larvae for my thesis. Do you know any website that sells or any lab that produces Lymantria? Thanks.

01 April 2015 5,900 17 View

Is there anyone who works with Bacteriorhodopsin?

24 September 2014 3,565 4 View

What is the pH effect on UV spectra?

Could you please give me some information about the pH effect on the UV spectra? Is there a common tendency for pH changes or does it depend on the molecule which it is measured? Thanks in advance.

07 September 2014 6,970 15 View

From particles number to concentration - can anyone help?

02 September 2014 6,750 19 View

No title

I would like to know how you guys use AIBN for cross linking? Should I just mix a certain amount of the monomer and AIBN and let them interact at a specific temperature? What are the tips for the...

09 April 2014 8,152 4 View

What are the reasons that polymers (not conducting) interact with double stranded DNA while not interacings with single stranded DNA?

Polymer and DNA interaction

08 January 2014 7,628 3 View

How can we understand if a polymer is conducting or not?

I have a polymer which hasn't been studied before. I did some cyclic voltammetry studies to evaluate it has oxidation or reduction potential or not. I found that this polymer has oxidation polymer...

08 October 2013 6,936 11 View

Best hybridization buffer for DNA Hybridization?

I would like to use hybridization between a probe and target DNA. We usually use PBS Buffer as a hybridization buffer. I want to try another solution. Any recommendations?

30 September 2013 1,519 30 View

Can someone advise on how to calculate detection limit of an experiment related to probe concentration? Is there any formula?

21 July 2013 2,456 6 View

What is the PCR amplification of specific alleles (PASA) method?

For PASA Method, specific primers are generally used. What is the point of designing primer? What should be considered when designing primers?

24 February 2013 1,450 1 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

What solvent can I used for Paal Knorr reaction between 6-amino hexanoic acid and 2.5 hexanedione?

I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...

02 March 2021 4,443 2 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

I want to performe MALDI-TOF MS for bacterial identification, I would appreciate it if anyone can tell me the matrix solution preparation protocol ?

based on what I've studied on multiple papers, for peptide and protein identification Alpha-cyano-4-hydroxycinnamic acid (CHCA) is an appropriate matrix to use, I also know that the CHCA powder...

26 February 2021 6,799 3 View

How to replicate the well conditions of a crystal hit with 20%(w/v) isopropanol?

I had a crystal hit in a well with 0.1M HEPEs pH 7.5, 10%(w/v) PEG 4000, and 20%(w/v) isopropanol. Why is isopropanol in w/v here if it's just solvent? How do I recreate this well condition?

25 February 2021 9,761 1 View