I am trying to look for the presence of larvae in artificially digested tissue samples.
Dear Cyrelle Besana
Yes, despite its small size (less than 500 one), the larvae (L3) of Toxocara can be seen in a stereomicroscope, even in the smallest magnification.
However, I think the use of a concentration technique, such as Barman-Moraes, can help you in the larval recovery.
The morphology of the larva can be seen in the attached figure.
Good luck in your study
Best regards,
Hudson
Although it is possible, in practice it is not advisable looking for Toxocara canis or T. cati larva under stereo microscope specially when you are looking for infected paratenic host tissues. In that case it is better to digest the tissues and look at under low power microscope for larva.
Rountinely, I'll observe the viability of T. canis larvae cultivated in RPMI under dissecting microscope. Certainly, artificial digestion of the tissue to liberate the encapsulated larvae is the common method and the larvae can be observed under dissecting microscope; but for saving the time you may utilize the centrifugation method to concentrate the larvae that will be easily for you to observe those larvae after digestion of the tissues.
I agree with Prof Sadjjadi, you couldn't differentiate between the larvae of T. cati and T. canis under stereo microscope.
Yes, Toxocara larvae are clearly visible under the dissecting microscope in artificially digested tissue samples. The digests were filtered through a sieve. The sediments were centrifuged and examined for Toxocara larvae
Yes, after the digestion and centrifuge the tissues, you can see the larvae under the stereomicroscope (the morphology of the larvae can be seen, as Dr. Pinto mentioned to it).