Are there reliable inhibitors for MARK2 in human cells?

More Viji M Draviam's questions See All

Why we are getting pattern 1:2:2:1 for DMPO-hydroxy radical in electron spin resonance? Reason?

How electronic energy diagram resulting from the interaction between DMPO and hydroxyl radicals ?

08 April 2019 132 2 View

Is there any reference about the amount of H2O2 present inside the cancer cell?

What could be the quantitative amount of H2O2/g of protein present inside the cancer cell?

24 March 2019 2,011 3 View

How to confirm magnetic nanoparticles coat with APTES is successful or not? Is it possible to know how much % coating happened?

In the synthesis of TEOS coated followed by amine functionalized nanoparticles magnetic nanoparticles using APTES, how to know amine group is functionalized? Is there any specific peak for APTES...

14 August 2018 8,787 3 View

How to solubilize chitosan in aqueous media? Does chitosan can be solubilize in any organic solvent?

18 October 2017 7,218 3 View

Can anyone give the procedure for estimation of metolachlor residues in plants?

agriculture scientists

31 March 2015 5,220 1 View

How can I seed cells onto a collagen sheet?

I m using collagen impregnated with plant extracts for my studies. I want to check biocompatibility of the material using MTT assay. Can anyone suggest me a detailed protocol for the same?

07 February 2015 4,143 5 View

Can I use DMEM medium without sodium pyruvate for culturing RAW 264.7 cell lines?

I am currently working with RAW cell lines. For passaging I am using DMEM medium w/o sodium pyruvate. Will it cause any problem during passaging? What is the importance of having sodium pyruvate...

23 January 2015 3,922 2 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

What element is driving the different slop in the low frequency tail?

My study consists simple corrosion inhibitors in salty brine. Right now I just fit my circuit with randles with CPE. Just wondering how the slop can given more info of the experiment. Or any...

01 March 2021 6,474 3 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

Daniel Mueller

There is one inhibitor available from Calbiochem which is described as MARK2 specific (MARK/Par-1 Activity Inhibitor, 39621 - Calbiochem, Timm, T., et al. 2011. J. Biol. Chem. 286, 41711.) but I have no first hand data about its reliability in human cells.

Depending on your experimental requirements, you may also use a general protein kinase inhibitor like staurosporine which inhibits MARK2 in-vitro with nanomolar potency. But due to its unspecificity this inhibitor is not really well suited for cell culture experiments.

Goodwin G. Jinesh

GSK3b inhibitors might help. LiCl, CHIR etc.

Viji M Draviam

Thank you all for the suggestions. we will try the Par1 inhibitor.

Viji