07 February 2024 1 1K Report

Are there regulatory requirements for the design and organization of rooms for clinical NGS laboratory?

More Anton Chechushkov's questions See All
Alternatives to traditional cloning of insert into backbone (dsDNA)?

Hi, I am stuck with a cloning experiment: The insert & backbone(bb) share one compatible end at 3' end(NotI) and one incompatible end at 5' end (XbaI for insert; HindIII for backbone). So i...

16 February 2021 1,664 3 View

How structure of monomers in molecular imprinted polymers influence their selectivity?

Hello, I'm biologist and currently working on MIP. I wanted to know is there any literature that mention the influence of monomer structure (for example size of molecule) on MIP selectivity and...

27 October 2020 1,571 6 View

What mask can be used for ultra-deep KOH etching on 110 Si?

Hello everyone! I need to etch Si to the depth of 1mm approximately, not affecting the surface of the sample (which is atomically flat and must be kept as it is, so we can't anneal the sample)....

16 August 2020 9,727 1 View

Are there any papers where advantages and disadvantages of different drug delivery systems are observed?

I'm writing PhD thesis and need to compare different drug delivery systems applied to SOD delivery.

22 July 2020 7,210 2 View

Which molecular structures are stained by trypan blue and ink-vinegar for visualization of mycorrhiza?

What do these substances stain exactly?

20 June 2020 8,653 1 View

How to calculate the band structure for a two-dimensional triangular lattice?

You can provide links with calculation and clear examples of the methods used. I would also like to understand how to take into account a specific potential. How to determine the forbidden zone...

09 June 2020 9,728 2 View

Can anyone advice empirical papers on ideas and their impact on institutional change?

Does anyone know any good papers on the impact of the political elite's ideas on institutional transformations in terms of comparative politics? It will be really great if there are both...

27 February 2020 3,665 3 View

Could someone recommend a journal similar to Biodiversity Data Journal?

I'd like to publish some ideas about taxonomical database. This is possible to do in Biodiversity Data Journal or PeerJ, but I need to pay for an article. Does anybody know similar journals...

23 February 2020 6,876 3 View

Is "green energy" really "green"?

Is "green energy" really "green"? Is Green Energy Really Eco-Friendly? Isn't solar panel manufacturing too dirty? Is it not too damaging to put wind turbines in farmland? Don't we pay too much...

24 January 2020 4,067 14 View

Is it possible to purify the protein using Ammonium Sulfate precipitation only?

Dear All, I am about to start purification of many variants of not tagged recombinant protein expressed in E.coli. I need it to be at least 70% pure. Column purification would be very time...

09 January 2020 7,116 4 View

Similar questions and discussions
Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Samples to study circular dichroism (chirality)?

I want to build a low-cost polarimeter to study circular dichroism (chirality). What type of samples can I use? I am looking for samples that can be found outside the laboratory.

01 March 2021 9,101 3 View

How to accurately quantify chitin?

Hi all, I have been working on the quantification of chitin in insect samples and feed for a while but I can notice that the quantification methods (e.g. exposure to HCl, NaOH, and weighing sample...

01 March 2021 1,843 2 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Can anyone help with Schistosoma identification?

Can anyone identify what kind of Schistosome species this might be? Morphological features of cercariae and adults resemble Trichobilharzia, though I am fairly new to the field of parasitology...

25 February 2021 7,760 6 View

How do you mesh a thin solid in Fluent?

Hello, I have used the shell function on SpaceClaim to create thin solids for the model I am studying. These are contained within an enclosure for my study. I chose this approach as it avoids the...

24 February 2021 8,856 3 View

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