Not satisfied with the inhibitors we used before,I am looking for alternative potent and specific inhibitors for caspase-9 and caspase-3. Let me know the catalog number. Thanks.
hi
from which company u r currently using. In our lab we use from sigma at 20 uM concentration and they are working fine. Which method u r using to evaluate the efficacy of inhibitors?
R&D systems Caspase-3 inhibitor z-DEVD-fmk (FMK004) (used at 20 uM). Blocks caspase-3 substrate cleavage by WB.
R&D systems Caspase-9 inhibitor z-LEHD-fmk (FMK008). I haven't tried it before. Should work at 20uM.
Hi, Mohammad,
We stain the cells with Annexin V and PI(or other DNA stain) to evaluate apoptosis.
How about using the pan-Caspase inhibitor ZVAD-FMK? We're using it in our lab at a concentration of 50 µM in order to get information about cell signaling independent of Caspase-induced cell death.
HI, Goodwin and Mohammad,
I googled online yesterday. Seems that z-DEVD-fmk and z-LEHD-fmk are the most widely used inhibitors for Casp-3 and Casp-9. I ordered them from BD Biosciences yesterday.
Thanks for your answer!
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