Rongqing Aaron Pan

4 Questions 15 Answers 0 Followers

Questions related from Rongqing Aaron Pan

Representative HUMAN Breast Cancer, Lung Cancer, Melanoma Cell lines?

I am planning to do a very preliminary experiment using a few representative solid cancer cell lines. Probably Three for each cancer types. For Breast cancer, I am thinking to use MDA-231, MCF7...

04 May 2018 4,329 4 View

Are there leukemia cell lines, especially AML cells, derived from the C57BL/6 mice?

Syngeneic AML mouse models.

02 January 2018 6,860 3 View

Do you snap freeze your cells and make cell lysates later to check phospho-proteins by western blot?

I am working with leukemia/floating cells. Typically, I spin down the cells, wash them with ice-cold PBS, spin again and then lyse the cells immediately. I am thinking If you could snap freeze the...

31 August 2015 6,533 13 View

Any suggestions on markers for cell cycle arrest (e.g. G1 arrest)?

I have been using Edu/Brdu + PI to analyze cell cycle status. This method can well distinguish cells in G0/G1, S, G2/S with zero/minimal overlapping. After treatment, cells in G0/G1 phase could be...

21 April 2015 5,777 10 View