Can I inactivate restriction enzymes after freezing the digestion product?

I performed a digestion that ended up at midnight, but forgot to inactivate the enzymes (HaeIII and RSAI) before freezing the products. I plan to run a gel to differentiate yeast isolates. Can I...

09 December 2020 3,551 3 View

Frozen spheroid section is not staining (fluorescent + H&E), any ideas?

I have cryosectioned a spheroid which was grown in collagen and agarose and then embedded in OCT Tissue Tek solution before freezing in a dry ice ETOH bath of liquid N2. The sections are very...

08 November 2020 3,299 3 View

Anyone with experience on DNA extraction from biopsies for microbiome analysis?

We shall collect biopsies from skin/hoof of animals for bacteriological microbiome analysis. We need suggestions on DNA extraction kit that may be used for this. We prefer to keep the biopsies in...

24 September 2020 6,845 4 View

How can I remove an article I have uploaded with a wrong title?

24 February 2020 6,806 3 View

Has anyone used a Phalloidin stain that permeates the cell membrane of living cells (rat cardiomyocytes)?

In this particular experiment, it is not possible to perform any permeabilization protocol that weakens the sarcolemma or cytoskeleton, so I would have to rely on finding a Phalloidin that can...

09 January 2018 2,642 4 View

Any tips in conducting the sulfide method using the 'Standard methods for the examination of water and wastewater'?

23 January 2017 3,448 3 View

How do you calculate N2O and CO2 flux emissions collected at different time? What equation(s) would you suggest?

This is a soil incubation experiment in situ. Collection occurred throughout a period of time. Please provide ideas of one or more equations that you could suggest to transform my values into...

21 September 2016 6,857 4 View

Are there any other tracking methods aside from satellite tagging that are just as efficient in tracking the long distance movements of whale sharks?

The migratory patterns of Rhincodon typus is very interesting to me and I have read that satellite tagging is the method typically used when tracking their movements. However, I would like to know...

07 July 2016 3,382 2 View

How to deal with mixed methods studies in mixed methods synthesis?

I have been reading about mixed methods synthesis techniques (Sandelowski et al 2006, 2012 and Joanna Briggs Institute 2014) for systematic review. I think a segregated synthesis approach will...

05 June 2015 138 5 View

What is the minimum fractionation method that is needed for whole mammalian cell lysate prior to Orbitrap-MS?

01 February 2015 9,914 5 View

How to code open-ended questions in SPSS?

Hello all, In SPSS I am going to code 2 open-ended questions. I have already read all the answers and I made a list of the most important categories to which I can code the answers. This question...

02 March 2021 1,757 4 View

How to eliminate Steady state error from Reinforcement learning genetic algorithm code?

The following code (see 1st 2 images attached) is used to produce PID controller values that are designed to control the system (G). The code finds the PID controller values (noted as k) by using...

28 February 2021 6,560 14 View

How could I process data in RCT to include it in meta-analysis?

I'm in the process of doing a meta-analysis and have encountered some problems with the RCT data. One of my outcom is muscle strength. In one study, I have three different measurements of muscle...

25 February 2021 7,603 3 View

Satellite orbit estimation using Recurrent Neural Network - what should I use?

Hello, I am a master's student studying in Yonsei Univeristy, Korea. I am trying to estimate the state of satellite, using Neural Network. Below is a simple flow of my study. 1. Train (t0 ~ t1)...

23 February 2021 6,383 3 View

Any Differences in COVID-19 vaccines?

Are there any differences (outside of the clinical trials that led to their EUA) observed in the rate of infection among those who have received the Pfizer vs Moderna vaccine since the mass...

22 February 2021 9,727 6 View

How can I get the coding gene sequences of Hela and U2OS cell lines? Is mRNA sequence of Hsp90 alpha tissue specific??

I am looking for hsp90 mRNA sequence in Hela and U2OS cell lines, I have tried through ncbi but always the output is hsp90 in homo sapiens not in the specific cell line. Kindly, could any one...

22 February 2021 1,853 3 View

How to constrain genetic algorithm input u?

My real system (buck converter) can only take an input of 0 to 1 (duty ratio) and I need to constrain the system so the controller action keeps within this bounds. How can I do this? The code for...

22 February 2021 2,125 3 View

Difficulty in debugging code to correct error - TypeError: '<' not supported ?

I am trying with a Unet code ontained from net ...in tensorflow. When trying to predict the segmentation result with the trained and saved model with metric " iou" using "CustomObjectScope"...

22 February 2021 5,254 1 View

Looking for advices and tips to improve the staining and the gating strategy in human blood?

Hi, I am staining a human blood with CD19, CD3,CD14 and CD16. My gating strategy in FlowJo goes like this: - First, I gate a general population SSC-A VS FSC-A => Then gate for single cells, =>...

21 February 2021 5,724 4 View

Has anyone used deep learning for 2D/3D REGISTRATION ?

I am a Master student in Biomedical (Bio-electrical) Engineering and work on " 2D-3D registration ( CT and X-Ray) as my thesis. I created DRR from CT and want use CNN( Convolutional Neural...

19 February 2021 6,354 3 View