They way I would propose is to take advantage of Copper-free Click-Chemistry. That means reaction of an alkyne group with an azide-group.

One way to take advantage of this is to couple the alkyne group to your thiols, e.g. by using (I10189) iodoacetamide alkyne.

And you order a N3-coupled-Oligo from your favourite provider (by-the-way we have an N3-Oligo-Atto647 Oligo and a fitting quencher at hand).

In principle this are two straight-forward reactions with easy to achieve purification.

Best,

Christian

Thanks a lot, Christian, for the information on this very interesting approach!

Best regards,

Markus