I am trying to determine whether the presence of monocytes/macrophages in my tissue is from infiltration or proliferation/maturation of a resident population.
for ideas... especially using the parts with BrdU and figure 8E/F.
3. Also, if you know that your macrophages are radioresistant in the tissue of interest, more complex parabiosis experiments/BM reconstitution experiments may be possible...
see http://jem.rupress.org/content/210/10/1977 for an example...