Can someone suggest a way to prepare slides to observe under confocal microscope for observing fluorescent tagged proteins??

I am growing the cells on coverslips and transfecting the fluorescent tagged protein directly on them. Then i want to observe these cells under confocal microscope. Currently i am just using...

01 March 2021 6,142 3 View

What are the different subclasses in intermediate seeds?

I am currently working on an endemic tree species, that produces mature seeds (moisture content 51%) which shows both vivipary (30% of total harvested seeds from a tree) and dormancy (~40%) of...

20 January 2021 2,670 3 View

Do you can put again my publications online?

Dear All, Please I has accidentaly removed 22 publications from RG my profile in the mail that you send me today, please put again those publicatios in my profile. Best regards,

13 December 2020 9,981 4 View

Adhesion energy (MOF-water) >> cohesion energy (MOFs) results higher uptake- how can I prove it thermodynamically?

In a surface energy analysis, we have found a very interesting result- the cohesive energy of aluminum fumarate is lower than the adhesive energy of aluminum fumarate-water pair. Also, the uptake...

05 November 2020 4,292 2 View

How can one automatically extract drainage basin values from a DEM following Strahler stream ordering approach?

Analysing hydrologically corrected DEM avails an opportunity to calculate morphometric indices such as drainage basin density(DD) (which takes manual approach). Can not DD and others be extracted...

07 October 2020 3,893 3 View

How to hydroxylate the surface of bismuth telluride nanoparticles?

Would it be possible to add -OH functional groups to Bi2Te3 nanoparticles?

07 October 2020 6,560 3 View

What is the chemical stability of bismuth telluride either in bulk or nanoparticle form (e.g., nanowires, platelets, etc)?

I am interested in post-synthethic methods of this material, and would like to know the chemical stability of Bi3Te2 (particularly nanotubes) in water, acid o alkaline media. Any literature...

03 October 2020 2,743 3 View

Does reducing the brain neuronal network as an inevitable aging event serve to consolidate memory tracking?

07 September 2020 6,188 2 View

What are all the criteria for the occurrence of Lunar Eclipse?

I have read the maximum limit of Lunar Eclipse is 12.2 degrees of arc (i.e. the difference in the longitude between Moon and any one node of the Moon). However on on June 5th 2020, a partial Lunar...

27 August 2020 986 5 View

Is it ok if in measurement model one of the construct having less AVE than 0.5?

In measurement model, one of the construct having AVE 0.4 but the composite reliability is 1 and Chi square = 1.099, GFI = 0.995, CFI = 0.997, RMSEA = 0.022. So, is it OK to go ahead in SEM.

17 July 2020 7,088 4 View

What is the status of bread making and more bakery foods with the use of insect flour/powder?

Insects have been consumed by human diet since prehistoric times. At present, the idea of looking at insects as food has been getting traction worldwide, also in response to increasing food...

03 March 2021 6,223 5 View

After a reference from Int J Body Compos Res, can any body help?

Hi, I am after the reference below, my library says it cannot obtain a copy either locally or internationally, any help appreciated! Chris Wang ZM, Heshka S, Wielopolski L, Pi-Sunyer FX, Pierson...

03 March 2021 6,193 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

How to grow E.coli on the low salt LB agar plate with 50 or 100ug/ml of Blasticidin?

Hello, I would like to ask how to grow E.coli which resists 50 or 100ug/ml of Blasticidin. I have tried many methods by using low salt Luria-Bertain(LB) agar plate. However, no colonies grow. Let...

01 March 2021 2,027 2 View

Does anyone know to clarify these changes?

01 March 2021 1,290 2 View

1 nm thick MgO thin-film to be deposited on Si wafer using thermal evaporation. What will be the optimum deposition conditions?

Favourable conditions of base pressure, rate of deposition. Also, by which characterisation technique to be used to verify the thickness of the deposited film.

28 February 2021 3,640 3 View

In an eye with Tyndall, does each dot correspond to an individual cell?

When you observe the presence of Tyndall in an inflamed eye: Does each of the small floating dots correspond to an individual cell or to an aggregate of cells? Does a conventional slit lamp have...

27 February 2021 6,326 2 View

How to calculate the crystallinity of cellulose film?

27 February 2021 2,753 4 View

What could be the reason for not having further low chi square value (better agreement) in my refinement result?

I have tried every combination of parameter changes and after that i have a poor chi square value. I can't get it lower any further. I know there have been a lot of discussion about Rietveld...

26 February 2021 8,480 3 View

Can anyone share his experience regards Smoothing in origin?

I am working on TiO2 composite material and the results of XRD have a lot of small/junk peaks and while I apply a smooth filter in Origin then the other characteristics peaks disappear so anyone...

26 February 2021 8,183 3 View