Are there any tips for obtaining clear images (20x) of cell files in each zone of Arabidopsis roots?

I normally fix them first in 96% ethanol and mount them in chloral hydrate. However, due to the ethanol, the roots are wrinkled, starting at the elongation zone (the meristem is okay).

Reducing the timing of ethanol clearing does not alter this effect, nor does using a lower percentage of ethanol, as observed in my samples with GUS-staining which are conserved at 70%.

I could try increasing the percentage, but this will be time consuming to apply to each sample.

Does anyone have any other ideas?

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