I would like to know if there is a way to determine the state of activated microglia cells in culture? Does anyone know a staining or ELISA/qPCR (or any other) method in which you can show if activated microglia cells are activated in the M1 or M2 phenotype?
I thought there might be some specific protein or receptor expression on the two types.
I know that M1 is mostly pro-inflammatory and secretes IL-1b, IL-6, IL-12, TNF-a and the other anti, secreting IL-10..
The thing is, for my research it may be benificial to show if the cells have polarized towards the M2 state from the M1 state.
It appears that they can be distinguished by the expression of CD206 and FcyRII/III... see https://www.ncbi.nlm.nih.gov/pubmed/23928152.
Bests,
F
Hi,
you can read the following papers for important info-
1. Alzheimers Res Ther. 2015 Aug 19;7(1):56. doi: 10.1186/s13195-015-0139-9.
Immune phenotypes of microglia in human neurodegenerative disease: challenges to detecting microglial polarization in human brains.
2. Microglial M1/M2 polarization and metabolic states.
Orihuela R, McPherson CA, Harry GJ.
Br J Pharmacol. 2016 Feb;173(4):649-65. doi: 10.1111/bph.13139.
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