Are there any european suppliers of transgenic mice (BigBlue or Mutamouse) for in vivo genotoxicity assays?

Transgenic mice carrying lacZ or lacI loci are recommended as preferred in vivo models for genotoxicity studies. However, in a number of papers usign these animals commercial suppliers are not...

01 February 2018 3,659 0 View

Are there any human IgG tolerant mouse models freely available for research purposes and not for human mAbs production?

Recently, a paper by Bi, V. et al (J Pharm Sci. 2013 Oct;102(10):3545-55. doi: 10.1002/jps.23663. Epub 2013 Aug 7.) has been published describing the use of XenoMouse x B6 F1 (Xeno-het) for...

08 September 2015 3,606 5 View

Is there a method for comparing the dynamics of T cell response among various species?

I would like to compare kinetics of CD4+ T cell activation among various species (mouse, cow, pig, dog, cat). I wonder what common stimulation procedure to apply to these T cells (CD3, PHA, sAgs?)...

02 March 2014 6,052 3 View

How to stain proteins in solution with Comassie Brillant Blue?

I'm in need of a home made protein molecular weight marker for Western blotting.

31 December 2013 5,202 8 View

Are Brij or Chaps-based buffers more appropriate for co-immunoprecipitate cell membrane protein complexes from Jurkat?

We tried to follow a published protocol where authors claim they can co-immunoprecipitate protein complexes from lipid rafts with RIPA buffer (1% NP-40, 0.1% SDS) but we cannot reproduce their...

06 July 2013 4,372 5 View

Which of the Covid-19 Vaccines would elicit the best immunoprotection and why?

I want to know which of the available Covid-19 Vaccines would elicit the best immunological response and why

02 March 2021 1,388 3 View

No title

02 March 2021 3,060 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

What is the procedure to determine the permeability of concrete?

can anyone help me in this, it will help me with my research work

01 March 2021 834 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View