Arkadiusz Miazek

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Questions related from Arkadiusz Miazek

Are there any european suppliers of transgenic mice (BigBlue or Mutamouse) for in vivo genotoxicity assays?

Transgenic mice carrying lacZ or lacI loci are recommended as preferred in vivo models for genotoxicity studies. However, in a number of papers usign these animals commercial suppliers are not...

02 February 2018 3,702 0 View

Are there any human IgG tolerant mouse models freely available for research purposes and not for human mAbs production?

Recently, a paper by Bi, V. et al (J Pharm Sci. 2013 Oct;102(10):3545-55. doi: 10.1002/jps.23663. Epub 2013 Aug 7.) has been published describing the use of XenoMouse x B6 F1 (Xeno-het) for...

09 September 2015 3,651 5 View

Is there a method for comparing the dynamics of T cell response among various species?

I would like to compare kinetics of CD4+ T cell activation among various species (mouse, cow, pig, dog, cat). I wonder what common stimulation procedure to apply to these T cells (CD3, PHA, sAgs?)...

03 March 2014 6,096 3 View

How to stain proteins in solution with Comassie Brillant Blue?

I'm in need of a home made protein molecular weight marker for Western blotting.

01 January 2014 5,238 8 View

Are there any successful immunotherapies based on cell permeable proteins?

After an initial observation that HIV-tat protein derived peptides mediate an efficient cellular uptake of variable proteins, apparently there are not many clinical applications of this...

01 January 2014 3,603 2 View

Are Brij or Chaps-based buffers more appropriate for co-immunoprecipitate cell membrane protein complexes from Jurkat?

We tried to follow a published protocol where authors claim they can co-immunoprecipitate protein complexes from lipid rafts with RIPA buffer (1% NP-40, 0.1% SDS) but we cannot reproduce their...

07 July 2013 4,421 5 View