Gap junction might have role in process of gadolinium clearance from healthy/damaged myocardium
Hello, Do any of you have experience with the analysis of chest wall compartments using a 32-marker model? Currently, we are using the standard 89-marker model, which is very time-consuming. Is...
26 October 2023 9,124 1 View
Hello. This year we started using ITC in a bit unconventional way to study enzyme kinetics of beta-lactamase (there is already literature data that showed it works). The principle is fairly...
06 September 2023 1,801 3 View
Hello! I'm using Endnote online and need help finding a reference style similar to JSCR, as the JSCR official reference style is not in Endnote online. Can anyone help? Thank you!
24 July 2023 6,200 1 View
Each life form has its own viruses. I am wondering if Plasmodium has specific viruses as well and which could be utilized as a measure to reduce Malaria infections? A virus which infects a...
16 January 2023 4,922 2 View
Of course, phages do only interact with bacteria which have specific binding proteins. But why do only bacteria have these binding proteins? What if vertebrate cells would have these binding...
07 January 2023 1,583 4 View
Here is what I'm trying to do: to solvate a peptide in water and ions that would mimic a PBS solution. To do this I tried uploading my peptide into the Solution Builder, adding the following...
13 December 2022 4,331 0 View
Dear RG community, I'm starting a photoacoustic project, and I need to acquire some ultrasonic receivers. Acoustics is not my expertise, so I'm asking here for help! :D Where to buy, what is...
28 September 2022 4,408 6 View
Hi there, I am a student who is very new to ANSYS. I want to simulate the following scenario A flexible rod is connected to a rigid hub. The hub starts from 0 rad/s at 0ͦangle and reaches 100...
25 September 2022 1,560 0 View
The photos are from the inland of Sao Miguel - Azores.
22 September 2022 1,972 5 View
With ongoing development of real-time "Free Style Libre" insulin detectors, has anyone ever did insulin analysis over a week period for a wild "forest" mice (not wild type lab mice)?
15 August 2022 7,487 4 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
04 August 2024 1,200 2 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View
I would like to perform transfection with the reagent DharmaFECT Duo (Horizon) on the AGS cell line. Could you please inform me of the optimal concentration to use without causing cytotoxicity in...
03 August 2024 3,851 1 View
Hello, if you made a transwell assay where you incubate the cells with a nanoparticle-encapsulated drug and considering that in the oposite compartment you'll have both the free and encapsulte...
03 August 2024 2,730 1 View