Detection of DNA Damage in proliferating cells?

I want to detect that my proliferating cells underwent a DNA Damage after treatment with drugs. I already know the H2AX method but looking for other methods. Many thanks in advance!

16 August 2020 4,179 6 View

Is it better to use anhydrous solvents for electrospinning process?

I wonder whether it is better to use anhydrous solvents for electrospinning process or not. In addition, what is a good alternative for electrospinning of PLA instead of 1,4-Dioxane in terms of...

03 May 2020 9,797 2 View

How can I prepare PLA (poly lactic acid) solution for electrospinning?

I've used Chloroform/DMF binary solvent system to dissolve the PLA at different concentrations. However, I've experienced clogging and multijet at the tip due to the fast evaporation of the...

02 February 2020 5,578 7 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

What is the density for pva electrospining?

I'm Juan Carlos, a high school student who is working with electropinning, I made an electropinning machine with a 21g needle and a 30kv high voltage source and the distance between my needle is...

28 February 2021 298 3 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

No title

I am trying to find how to know the density needed for a pva electrospinning in a 0.6mm neddle, the electrospinning will be from top to bottom and the problem I really have is the solution because...

25 February 2021 2,615 3 View

Can I use acetic acid with an ELITE WAX Perkin Elmer column for Gas Chromatography?

Hi! I would want to know if I can use acetic acid with an ELITE WAX Perkin Elmer column for Gas Chromatography. This column has a polyethylene glycol phase, but I can't find the manual to check...

22 February 2021 730 2 View

How to seperate PEG-4000 from aqueuse phase ?

Hello everyone , Please tell me if It possible to seperat POlyethelyn glycol -4000 from an aqueuse media by other ways rather heating . I have tried salting out by electrolyte solution to...

22 February 2021 7,285 5 View

In what type of container can we put a 200 degree Celsius poly alkylene glycol? what should be the material of the container?

I am having a 200-degree Celcius heated poly alkylene glycol now I want to know in which type of container should I put that heated glycol or what should be the material of the container?

08 February 2021 6,819 1 View

Flexibility of RNAseq?

When you use RNAseq I understand that it must follow certain conditions when you use it. One of them is the insertion of a polyA tail. The insertion of the polyA must include then...

05 February 2021 3,770 4 View

Dear Colleagues, I am looking for a way to dop of electrospining nanofibers on the HEPA filter.Someone can guide me?

Electrospining method is important for production of nanofiber .If doped with nanoparticles ,can be used for remove of contaminant such as VOC , but don't have mechanical strengh . For finding a...

24 January 2021 7,290 3 View

GC-Headspace (HS-GC) chromatography for Forensic toxicological and Food expertises?

Dear Colleagues! How correct and appropriate is it to conduct forensic toxicological and food examination of volatile substances (ethanol, methanol, propanol, ethyl glycol, etc.) in blood and...

24 January 2021 5,645 2 View