I've been using a monoclonal anti-PSD95 for postsynaptic labeling in histological sections of rat brain, but the labeling is somewhat diffused. I would like to know any other markers of postsynaptic elements (in dendritic spines, if possible). Not for quantification, but for colocalization.
Tips and protocols for immunohistochemistry with anti-PSD95 would be useful as well (detergent concentration, antibodies used).
We have an IHC protocol on our website for IHC in rat brain: http://neuromab.ucdavis.edu/protocols.cfm . We are an NIH funded lab that makes antibodies for neuroscience research and have two PSD-95 monoclonals (the newest to be released soon) and a PSD-93 antibody, along others that may be useful to you, but specific questions can be sent to our e-mail address which will be read by our Facility's Director and/or Assistant Director. I'm not just trying to push our antibodies. If we do not have one that will work for you, we may know someone who does.
Phalloidin binds F-actin, which since it's highly enriched in dendritic spines, labels spines in IHC. There are fluorescent conjugates of phalloidin commercially available. Spinophilin is also enriched in spines, and anti-spinophilin abs label pretty well in IHC too.
We have an IHC protocol on our website for IHC in rat brain: http://neuromab.ucdavis.edu/protocols.cfm . We are an NIH funded lab that makes antibodies for neuroscience research and have two PSD-95 monoclonals (the newest to be released soon) and a PSD-93 antibody, along others that may be useful to you, but specific questions can be sent to our e-mail address which will be read by our Facility's Director and/or Assistant Director. I'm not just trying to push our antibodies. If we do not have one that will work for you, we may know someone who does.
Assuming you are interested in excitatory neurons, I would suggest Shank, glutamate receptors (eg GLUR1), NMDA receptors (eg NR1) or Homer. Shank was our favorite postsynaptic marker when we couldn't use PSD95.
NeuroMab (posted by Lea above) is a great resource for antibodies and protocols.
Thank you very much for your answers. I'll check the neuromab site and the markers you mention.
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