Are there any polymerases which work above 95 degree centigrade?

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Satyendra Mondal

I use PCR Using Q5® Hot Start High-Fidelity DNA Polymerase (M0493) from New England BioLabs which has a higher denaturation temperature i.e. 98°C.

Subhash C. Juneja

No. That is denaturation temp 95 and above.

Taq polymerase to build the complementary strand is around 72

Katie A S Burnette

Tell your professor this is not an appropriate use of this website. You can easily get the answer to your question by reading the description of commercially available enzymes.