I want to assay amikacin (an aminoglycoside antibiotic) concentration in the presence of another drug which has a single amine group. I prefer not to use methods which use amine groups and also I prefer to use spectroscopy.
Amikacin has no UV chromofor so a direct spectroscopic is very unlikely.
Ph.Eur. uses HPLC/PED for this group of compounds.
If your sample is not pure some cleanup/separation will be unavoidable
As you mentioned, Ph.Eur. uses HPLC for this antibiotic but in some articles there are derivatization methods using amine groups. For example Diphthalaldehyde reagent can react with amine groups and make it possible to use spectroscopy for amikacin assay. But unfortunately this kind of compounds are not suitable for me, because the other drug I use simultaneously has an amine group too! I am looking for any other methods except HPLC because I have no HPLC equipment right now.
You can refer following articles
1. http://www.japsonline.com/admin/php/uploads/836_pdf.pdf
2. http://ajse.kfupm.edu.sa/articles/352A%20p.02.pdf
3. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC352826/
Thanks
Imran
If your purpose is only to find the concentration of amikacin in your sample, you can use the agar well diffusion method chosing a test microrganism which is sensible to amikacin but resistant to the other antibiotic. This if you already know that the second antibiotic didn't interfere with the antimicrobial activity of amikacin. Otherwise, you can use FPIA (Fluorescent Polarization Immuno Assay) but this can only be done if you have instruments such as TDx or AxSYM
Tommaso Della Bora
I could not find such microorganism, it seems there is no microbiological assay for amikacin but generally it is a good idea, thank you so much.
For testing amikacin you can use Bacillus subtilis or Staphylococcus sp., but I don't know how they can behave with the other antibiotic.
Tommaso Della Bora
I will check them as soon as possible.
Thank you