How to avoid ulcers in s.c. injected tumors ?

Recently, we have injected s.c. B16F10 tumor cells in BL/6 mice. At around 12-15th day some animals showed ulcerations in tumors , with a big hole in the middle (it looks like a volcano). We have...

01 March 2021 9,938 3 View

What is the standard protocol in doing replicates for C. elegans lifespan assay on solid media (thru worm picking)?

I would like to know what is the standard protocol in the number of worms per plate and number of plates per concentration of extract X in a lifespan assay done in solid media and not automated...

23 February 2021 9,933 1 View

SPSS AMOS: Running my structural model decreases the CFI significantly - trying to account for it results in an unidentified model. What can I do?

Hi, after a thorough EFA and CFA, I've created my structural model (with imputed factor scores) in AMOS and ran it. While it provided me with all path coefficients, I found that the CFI had...

06 January 2021 5,087 4 View

What is the recommended/optimal time for resting mouse splenocytes after thawing?

This is my first time working with mouse cells. We isolated and froze down in 90% FCS and DMSO. I would like to retrieve them and do immunophenotyping and intracellular cytokine staining

03 January 2021 6,399 1 View

Does anyone know how to fix the "A sample of parameter values was inadmissible"-error in SPSS AMOS when imputing factors?

Hi all, I'm trying to impute factors after testing my model for CMV and therefore included a common latent factor. Unfortunately, I always get the same error: "A sample of parameter values was...

02 January 2021 6,647 5 View

Can CMOS and CCD images sensors be used as microphones?

I was thinking about Msps and Gsps image sensors in different environments. If a pair of identical image sensors - one exposed to air, gases, plasmas, or partial vacuum - and one in a good...

21 December 2020 9,747 2 View

Is there a tool to find the closest species with an assembled genome for any given species?

As more and more genomes are being sequenced the likelihood of having a close relative being sequenced is rising. So lets say I have a non-model species Chela cachius and I want to find the...

25 November 2020 6,267 4 View

How can I measure the bacterial growth in a wet fabric in a petri dish?

I have to measure the growth of the bacteria in a piece of fabric for my senior design project. I don't know how to measure it. The only way that I know is by the naked eye or by the microscope....

23 November 2020 2,897 6 View

Where can I find a longterm global mean EVI/NDVI dataset?

I am looking for a longterm (10+ years) dataset of mean EVI/NDVI or annual maximum EVI/NDVI and similar. Basically, I am trying to avoid downloading tons of images and doing the averaging myself,...

28 September 2020 8,374 2 View

How can we use satellite images to detect water quality?

Hi! We are high school students and our topic for our research subject is about mapping water quality using satellite images. How can we utilize satellite images (like using the color of a certain...

26 September 2020 7,072 7 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

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03 March 2021 5,360 2 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

CGA 300 Regulator for CO2 cylinder?

Has anyone ever tried to deliver CO2 with a CGA 300 regulator? I know that a CGA 320 regulator is standard for CO2. The issue is that I currently do not have any free CGA 320 regulators. I also...

25 February 2021 3,292 3 View

Sample size determination for non-parametric tests?

How do I run a a priori sample size determination for non-parametric tests, like the Mann-Whitney U-test and the Kruskall-Wallis or other non-parametric tests that accord with the unpaired t-test...

24 February 2021 166 6 View

Does PHA-P stimulate the proliferation of lymphocytes from mice?

Hello, I will start performing MLR using lymphocytes from mice's spleen and I would like to know the better way for the stimulation. If is using PHA-P or CD3/CD28? I would like to know as well if...

23 February 2021 5,476 4 View

I see 2 random bands in my ERK/pERK blot? Has anyone else seen this?

I ran a western blot using 12% gel and probing for ERK/pERK (cell signaling antibodies). The green is total ERK, the Red is supposed to be phospho-ERK. I see 2 bands below the total ERK that...

22 February 2021 1,777 3 View

Statistical Tests for Quantitative Study?

Hello Everyone, I have one population, sample size 200. and needed to find the correlation between Variable A and B. Also, which T-test should I choose for hypothesis testing Please advise...

21 February 2021 7,591 4 View

Looking for advices and tips to improve the staining and the gating strategy in human blood?

Hi, I am staining a human blood with CD19, CD3,CD14 and CD16. My gating strategy in FlowJo goes like this: - First, I gate a general population SSC-A VS FSC-A => Then gate for single cells, =>...

21 February 2021 5,724 4 View