I want to find patterns in proteomic data from an analysis of bacterial membrane proteins
Can anyone tell me the largest number of proteins directly extracted from soil ? I am writing a paper about soil protein extraction and wanted to know what sort of yields other researchers were...
31 December 2019 8,094 2 View
We are looking for a Candicidin D standard for HPLC and mass spectrometry. It is no longer made by the company listed on PubChem (alpha chemistry). Does anybody have any other suggestions?
31 December 2018 4,426 3 View
I am conducting a metagenomic analysis of alkaline soil and I wondered if there were any specialised methods of DNA extraction given the alkaline nature of the soil.
07 August 2018 3,257 4 View
I have been characterising a new strain of Streptomyces and was wanting to compare it to other similar alkaliphilic strains of Streptomyces. I have read of figures as high as 11.5 but the...
06 July 2018 1,556 8 View
I am being asked to provide polyphasic studies to name a new bacterial species, however, I have already provided the whole genomic sequence together with some comparitive genome analysis and some...
06 July 2018 2,071 0 View
Are there any references to the maximum pH levels tolerated by strain S. coelicolor M145 ?
03 April 2018 2,601 4 View
I had previously made a series of (modified SFM) agars at high alkaline pH 8----->13.6. I did not measure the pH of the resulting solidified agar because it just seemed a bit awkward and I...
02 March 2018 862 4 View
I was wondering if the pH of an environment influences the development of amyloid proteins ? Cellular control of pH is performed by porters/ antiporters. I have searched for these in human...
10 November 2017 9,375 4 View
We have sequenced a bacteria and found multiple copies of antibiotic resistance genes. The sequences of the copies are not identical. I was wondering if this diversity of copies is to increase the...
09 October 2017 6,473 13 View
I have been using activated carbon columns to clean up protein samples. Protein recovery has been very low. When I use a large amount of protein i.e. 5mg the recovery is only 0.5mg . I was...
09 October 2017 501 1 View
I would like to learn more about SPSS and Its application especially in regards to data analysis. Please suggest me how I can learn more about it. Thank you so much.
11 August 2024 9,101 4 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
Can anyone explain this method? Especially the last statement where it says only at 1.5 to 2.5mins was the MS/MS connected to the UPLC. How is that possible, is it a feature in this specific...
11 August 2024 8,141 3 View
Hello, Why do i see this baseline drift when i compare my blank (black) to the sample (blue)? Any suggestions as to why this happened? Thank you!
11 August 2024 3,770 4 View
Willett, Shenoy et al. (2021) have developed a brain computer interface (BCI) that used neural signal collected from the hand area of the motor cortex (area M1) of a paralyzed patient. The...
10 August 2024 7,180 0 View
I'm currently exploring the application of Python in textile engineering, specifically in areas like data analysis, process automation, and the development of smart textiles. I'm interested in...
10 August 2024 7,429 2 View
How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?
09 August 2024 7,718 0 View
Hello experts, Does anyone know any free software about retention index prediction ?
08 August 2024 7,403 2 View
I'm currently working on calculating the collision cross section (CCS) for various ions, and I'm facing challenges when dealing with sodiated and multiply charged ions. Most of the resources I’ve...
08 August 2024 8,329 0 View
Let's say we have a standard, regular hexagonal honeycomb with a 3-arm primitive unit cell (something like the figure attached; the figure is only representative and not drawn to scale). The...
07 August 2024 1,937 1 View