I want to find patterns in proteomic data from an analysis of bacterial membrane proteins
Can anyone tell me the largest number of proteins directly extracted from soil ? I am writing a paper about soil protein extraction and wanted to know what sort of yields other researchers were...
31 December 2019 7,930 2 View
We are looking for a Candicidin D standard for HPLC and mass spectrometry. It is no longer made by the company listed on PubChem (alpha chemistry). Does anybody have any other suggestions?
31 December 2018 4,280 3 View
I am conducting a metagenomic analysis of alkaline soil and I wondered if there were any specialised methods of DNA extraction given the alkaline nature of the soil.
07 August 2018 3,112 4 View
I have been characterising a new strain of Streptomyces and was wanting to compare it to other similar alkaliphilic strains of Streptomyces. I have read of figures as high as 11.5 but the...
06 July 2018 1,383 8 View
I am being asked to provide polyphasic studies to name a new bacterial species, however, I have already provided the whole genomic sequence together with some comparitive genome analysis and some...
06 July 2018 1,916 0 View
Are there any references to the maximum pH levels tolerated by strain S. coelicolor M145 ?
03 April 2018 2,449 4 View
I had previously made a series of (modified SFM) agars at high alkaline pH 8----->13.6. I did not measure the pH of the resulting solidified agar because it just seemed a bit awkward and I...
02 March 2018 710 4 View
I was wondering if the pH of an environment influences the development of amyloid proteins ? Cellular control of pH is performed by porters/ antiporters. I have searched for these in human...
10 November 2017 9,237 4 View
We have sequenced a bacteria and found multiple copies of antibiotic resistance genes. The sequences of the copies are not identical. I was wondering if this diversity of copies is to increase the...
09 October 2017 6,310 13 View
I have been using activated carbon columns to clean up protein samples. Protein recovery has been very low. When I use a large amount of protein i.e. 5mg the recovery is only 0.5mg . I was...
09 October 2017 357 1 View
How to calculate the RMSD values for a MD simulation using MOE?
07 August 2021 0 0 View
When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.
16 June 2021 0 0 View
Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...
03 March 2021 8,796 3 View
I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance
03 March 2021 3,568 1 View
Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...
03 March 2021 5,978 5 View
What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...
03 March 2021 1,401 3 View