What are simple and effective techniques for measuring the amount of Dye loaded in a photoanode of DSSCs?

I need to calculate the amount of dye loaded in a mesoporus semiconductor architecture. Please suggest the best way to find the adsorbed dye estimation. Thanks in advance!

31 December 2017 1,146 3 View

How to calculate aspect ratio of a nanorod from FESEM image?

07 August 2017 2,805 3 View

How to prepare redox electrolyte for DSSCs?

which is the best combination of redox electrolyte for DSSCs? ex. Li/I2 solvent proportion, chemical composition and preparation techniques

03 April 2017 575 1 View

Visible green emiting semiconduction nanoparticle. Will anyone suggest the materials belongs to this regime?

I need a semi conduction nano particles having emission 550-600 nm range beyond gold, ZnS and PbS. please suggest.

09 October 2016 3,847 0 View

Will Sodium dodecyl sulfate break and denature the structure of the proteins?

09 October 2016 9,981 3 View

Saturable absorption to reverse saturable absorption for a same material how it works?

In Z scan Experiment same material exhibits both RSA and SA, both phenomena are different any one can suggest me or send some texts to understand that. Thanks in advance...

06 July 2016 1,618 3 View

How can I fix the purity of the purple membrane obtained from Halobacterium salinarium?

04 May 2015 3,945 3 View

Does anyone have experience in calculating crystallite size using (scherrer calculator)X'pert high score plus software?

I am little bit struggled with calculating crystallite size using this software, I want to check with manual calculation. If tutorial available please send me

01 February 2015 6,393 1 View

Does anyone have the culture of halobacterium salinarum strain S9?

I need bacterioruberin free halo bacterium strain if its available please give me microbial collection center name... it will helpful to my primary work

31 December 2014 1,824 2 View

Which fluorescent dye to use for measuring total ROS production?

Is DCFDA appropriate for measuring total ROS production, or can it only detect certain types of ROS? If I want to measure total ROS production, which dye should I use?

01 March 2021 9,447 3 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View

In methylene blue test Why is UV absorbance reading very low as soon adsorbent is added and then increasing before reaching equilibrium?

Hello, I'm working on removal of methylene blue. 01. in the dark test very first reading is taken 15 minutes after adding adsorbent to the dye solution and that UV absorbance reading is very...

19 February 2021 472 5 View

Has anyone performed a colorimetric RT-LAMP assay using Lucigens LavaLAMP kit?

Hi there, my lab recently opted to use LavaLAMP from Lucigen instead of the Warmstart kit from NEB. However, Lucigen has yet to create a colormetric kit for RT-LAMP, and adding phenol-Red (pH...

17 February 2021 5,399 1 View

Why stop the reaction in ice for the fluorescent probe FDA (fluorescein diacetate)?

Hi, In literature I noticed that who stains haemocytes with FDA for 30 min then stop the reaction in ice while this does not apply for 15 min incubation time (or maybe authors did not write this...

08 February 2021 3,366 3 View

What is the possible cause of MSCs low growing?

Hello Everyone, We are inexperienced in culture of MSCs trying to grow a sample from BM of a mare. It is the second time that after preparation of new medium (DMEM-F12 with high glucose - 10% Hy...

07 February 2021 9,989 7 View

Why do cells die after adding MTT dye?

I was doing MTT assay, and in the last day before adding the MTT; the cells looked good (or so I think), but after aspiration of the drug and addition of MTT dye I noticed that most cells seemed...

07 February 2021 3,846 4 View

How to measure the concentration of a labeled protein with no tryptophan or tyrosines?

Hey I'm trying to measure the concentration of a protein I've purified and then labeled with Atto 647 flourescent dye. The protein has no tryptophans or tyrosines (although does have...

04 February 2021 4,482 4 View

Why are my western blot bands so distorted?

Hi guys I'm out of ideas here please help! I've never had consistent problem like this doing westerns before and I can't figure it out. The condition used: - First 4 lanes have 50ug protein in...

04 February 2021 2,297 3 View

How to remove unreacted florescent dye from labeled protein?

Hi I have a protein labeled with rhodamine B and after reaction i used Zeba spin column for dye removal. my problem is that lot of unreacted dye remained which interfered with my florescent...

02 February 2021 644 2 View