I am trying to calculate aspect ratio using image J software but not succeeded. Please suggest me to software’s and procedures to calculate the aspect ratio. The FESEM image is attached below which I have used for calculation.
image J software is enough to calculate the Aspect ratio= length/diameter of given nanorods. First of all, you have measure length and diameter at various place then will take average of those value. For example, your FESEM image scale bar 200 nm, first may calculate the average aspect ratio of given image, finally you can converted to 1X1 cm which means multiple value of 200 nm scale bar image to 1X1 image