Are there any interesting areas of research yet to be studied with rice viruses?

I'm currently in the process of coming up with a research pitch for a lab to be funded here in the Philippines. Rice is quite the common crop in the South East and its diseases are commonplace....

23 November 2020 5,194 4 View

What is the best method to conduct a meta analysis?

Dear Colleagues: For those of you who have conducted a couple of meta analyses, do you agree with these steps? What is the best method of extracting the data from the chosen studies? Formulate...

26 October 2020 1,539 6 View

Point zero charge Activate carbon, influence of mass?

Hello, I am going to do PZC of some activated biochar/carbon. I thought of doing it by Potentiometric titration with varying pHs and using a salt like NaCl at 0.01M. Now I am wondering: does the...

20 October 2020 5,108 5 View

Is there a database indicating the carbon and nitrogen storage capacity of vascular plants (in Europe)?

Hi everyone, I search a database or a review listing the capacity of vascular plants to storage carbon, nitrogen or other important chemical elements. I work actually on a mountain area (Swiss...

19 October 2020 8,596 3 View

Why is my Tet on system constitutively active and not dox inducible ?

I am using a Tet on system to overexpress a LncRNA in mouse ES cells, based on a piggybac vector (pXlone). I performed transient and stable experiments. In both cases, the LncRNA is expressed even...

13 September 2020 8,303 1 View

With increasing water shortage are the drought resistant plants going to be a solution for food production?

In many areas in the world the lack of water parallels the desertification.Is the creation of new drought resistant plants going to solve the problem for the world nutrition or do we have to...

20 August 2020 8,561 7 View

Have you found an arithmetic ratio of form 3/2 in your investigations in physics, quantum physics, genetic code, chemistry and other fields?

Do you think then that there is a universality of this particular ratio? I, for my part, highlighted this ratio in these different areas: Genetic code, quantum physics and the 3/2 ratio The...

19 August 2020 3,070 5 View

Generalization of a quantitative study?

I conducted a quantitative study addressing 340 executives. The administration of the questionnaire was held by a panel company. The analyses were conducted using Smart-PLS. A reviewer asked me:...

22 July 2020 7,099 2 View

Do anyone know a good software allowing interactive 3D visualization of density plot?

Hello, I would like to make a interactive 3D representation of some data point. We are studying position of some cell of interest and we extract from serial coronal section XY position of the...

15 June 2020 8,117 2 View

No title

14 June 2020 2,475 7 View

In what ways do fellow reaerchers think that mRNA vaccination may be under CRISPR systems technology application ?

There are many ways for mRNA to intervene with genome expression. This question is raised for experts in CRISPR systems. Any RNA does not stand alone within acell. There are many ways to become...

18 February 2021 9,109 5 View

How can I completely delete a protein on the genomic level using Crispr?

I want to completely delete or largely truncate a gene and not only achieve a functional knockout in a cell line. I can only transfect my cells with the Crispr/Cas9 sgRNA RNP and not introduce a...

08 February 2021 2,032 2 View

Choise of cell line for neurobiology?

I am working with myelin-related proteins and interested to do cell experiments with my target protein in cells. I have created lentiviruses for my proteins, and mutants and also CRISPR set-up for...

02 February 2021 8,083 1 View

Apart from MOFA what other software is available for analysis of a multi-omic study?

Study bringing together genomics, proteomics etc together

02 February 2021 2,856 2 View

Does gene insertion via CRISPR/Cas9 result in chromosomal abnormalities?

Hello, I hope to insert a gene into mammalian cells in culture using CRISPR/Cas9 and an HDR template. Will insertion of a 1 kilobase gene cause chromosomal abnormalities and effect cell...

31 January 2021 2,516 1 View

Is there any alternative to BUSCO for searching orthologues within genomes available?

I have a need to search for single-copy conservative orthologs within several plant genomes and used BUSCO tool for that. However, I found that the results produced by different versions on the...

29 January 2021 7,764 6 View

Does anyone has a protocol for transfecting AML12 cells?

I am optimizing a CRISPR/CAS9 system in vitro before using it in vivo. I would like to transfect AML12 cells with my pAAV-sgRNA-vector to validate editing efficiency before generating virus.

26 January 2021 8,733 2 View

How to convert ng/ul of DNA concentration to pmol/ul ?

Dear researcher, After amplifying my target gene (1900bp) into the required cDNA by PCR,>cut specific gel bands and purify the gel and the concentration was 30ng/ul. Next, performed A-Tailing...

21 January 2021 2,671 3 View

Can use Unigenes from RNA sequences for overexpression studies (transformation) without requiring a specific plant genome?

Dear researchers: If the plant genome has not been released yet, can Unigenes from RNA sequences be used after BLAST and confirmed with known Arabidopsis genes for function/overexpression...

20 January 2021 5,557 2 View

Should I add a promoter upstream of my CRISPR-inserted gene?

Hello, I want to insert a gene into my cells using sgRNA, Cas9, and an HDR template. The gene that I will insert into has its own promoter. Because I will cut the gene downstream of its start...

14 January 2021 1,170 2 View