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Questions related from Pierre-Yves Helleboid
I never worked with non-mammalian cell lines, I just ordered ZF4 zebrafish cell line and I am looking for a good expression vector and I have no idea which promoter would yield the most expression...
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I would like to know more about this technique in order to detect protein-protein interaction in 293T cells, is it hard to set up and optimize? What are the drawbacks?
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I have a library of tagged proteins overexpressing cells and I have total cellular extracts for all of them but in small amount (around 500 ng of total proteins for each) and I would like to avoid...
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I'm trying to KO several genes with CRISPR and I would like to have one donor plasmid for HR with a resistance gene and insertion sites for the homologous recombination arms.
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