Pierre-Yves Helleboid

5 Questions 3 Answers 0 Followers

Questions related from Pierre-Yves Helleboid

Zebrafish cell line ZF4 best expression promoter/vector?

I never worked with non-mammalian cell lines, I just ordered ZF4 zebrafish cell line and I am looking for a good expression vector and I have no idea which promoter would yield the most expression...

21 September 2018 8,480 1 View

Having difficulties for CRISPR in HUMAN iPS cells, any good reads with efficient protocols?

I am using CRISPR on human iPS cells to delete my gene of interest by using two guides that cut respectively in 5' and in 3' of the gene. I transfect with myrrus the two guides and the Cas9 and...

15 March 2017 9,104 1 View

Is Duolink an efficient technique to detect protein-protein interaction in the nucleus?

I would like to know more about this technique in order to detect protein-protein interaction in 293T cells, is it hard to set up and optimize? What are the drawbacks?

05 February 2015 5,559 3 View

What technology would you use to set up a large-scale protein-protein interaction assay?

I have a library of tagged proteins overexpressing cells and I have total cellular extracts for all of them but in small amount (around 500 ng of total proteins for each) and I would like to avoid...

02 October 2014 1,346 4 View

Are there any donor template plasmids with a pure resistance gene and on both sides restriction sites to insert the homologous recombination arms?

I'm trying to KO several genes with CRISPR and I would like to have one donor plasmid for HR with a resistance gene and insertion sites for the homologous recombination arms.

16 May 2014 3,148 4 View