4 Questions 3 Answers 0 Followers
Questions related from Pierre-Yves Helleboid
I never worked with non-mammalian cell lines, I just ordered ZF4 zebrafish cell line and I am looking for a good expression vector and I have no idea which promoter would yield the most expression...
21 September 2018 8,407 1 View
I would like to know more about this technique in order to detect protein-protein interaction in 293T cells, is it hard to set up and optimize? What are the drawbacks?
05 February 2015 5,522 3 View
I have a library of tagged proteins overexpressing cells and I have total cellular extracts for all of them but in small amount (around 500 ng of total proteins for each) and I would like to avoid...
02 October 2014 1,307 4 View
I'm trying to KO several genes with CRISPR and I would like to have one donor plasmid for HR with a resistance gene and insertion sites for the homologous recombination arms.
16 May 2014 3,118 4 View