Which dilution solution is the best? Or is there no differences between them?
In my experiments with E. coli, the cells kept in PBS at 6 oC multiplied overnight.
physiologic saline was used for Bacteria dilutions , It content 0.85 gm from NaCl in 100 ml D. water or pepton water using in Bacteria dilutions, It was prepared 0.1 gm in 100 D. water.
But phosphate buffered saline are found more types and used in more methods Exp. Enzymatic methods , DNA extract . The types of phosphate buffered saline was different in contents but no used in Bacteria dilutions .
See this link for a similar question;
Regards
Karen
https://www.researchgate.net/post/Which_one_is_better_to_be_used_for_dilution_PBS_or_physiological_saline
The best dilution solution is MRD - Maximum Recovery diluent. It is basically saline with 0.1 % peptone as a carbon source. Provides the perfect osmotic support necessary for small interval manipulation
I agree with Mary, saline without peptone may not be suffcient for the recovery of microorganisms because it may cause osmotic stress. Personally I use Maximum Recovery Diluent (Oxoid) - 0.45% NaCl and 0.45% peptone. If you need to recover microorganisms from feaces phospahte buffered saline should be better. You can check the description of the whole procedure in: ''Bacterial, SCFA and gas profiles of a range of food ingredients following in vitro fermentation by human colonic microbiota''.
Dear all, do you think the cells will multiplied in PBS or MRD since both of them does not contain any nutrients?
Physiological saline is not contemplated in the ISO standards as a diluent for microbiological analyses. Although it does not stress the cells from the osmotic point of view, it is not buffered. It is preferable to use PBS or peptone-saline.
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