Did any one have a micro array test for miRNA? I'm looking for a global or custom miRNA array kit?

A DNA microarray (also commonly known as DNA chip or biochip) is a collection of microscopic DNA spots attached to a solid surface. Scientists use DNA microarrays to measure the expression levels...

18 December 2015 9,279 3 View

What is the best method for identification of occult HBV patients?

03 September 2014 7,839 7 View

How can I assess the asymptomatic shedding of HSV in men and women?

Asymptomatic shedding of HSV is very important. After the initial infection of Herpes simplex virus, they hide in ganglia and are reactivate for a lifetime. However, some reactivation may be...

31 December 2013 5,842 1 View

Does the CMV re infect healthy people that already have been infected with other CMV genotypes?

We observed that rate of mixed genotype infection is much higher among older patients. I want to figure out the relation of age and mixed genotype infection.

15 August 2013 2,878 1 View

What is the most common number of epitopes in an multi-epitope Vaccine?

hi everyone, I'm interested in the field of vaccine design specifically multi-epitope cancer vaccines , I want to know if there is a minimum and maximum number of epitopes we could use in a...

27 February 2021 3,882 3 View

Different antibodies show signal at same MW in SDS-PAGE, what is wrong?

16 February 2021 2,857 9 View

Is there such a thing as Igg specific MHC class II epitopes or do all non-self MHC class II epitopes trigger antibody production of all subtypes?

I know there are bioinformatic tools that allow epitope prediction to determine if a certain peptide would trigger an immune response. I also read that for allergen diagnoses IgE of sensitised...

09 February 2021 4,173 3 View

How to characterize epitopes within a peptide sequence?

Greetings In a theoretical perspective, I want to use a human-derived peptide as a plausible antigen but the problem associated with tolerance hampers my approach. Then I thought why not denature...

20 January 2021 2,201 1 View

How can peptides based on allele sequences be delivered to mice?

I am looking to measure allele-specific immune responses for specific antigens. After identifying the most prominent alleles in a population, I am looking to express them in mice and then measure...

03 September 2020 9,281 1 View

MAb detecting two viral proteins?

Hi, What are the chances that the developed MAb can detect two viral proteins simultaneously? The MAbs were raised against purified viral particles. During the characterization studies, some...

28 May 2020 4,061 1 View

No title

11 December 2019 2,985 3 View

Has anybody experienced an ScFv that binds to an epitope of interest but binding is completely lost when cloned on to an IgG backbone?

While the epitope that I'm working on is a trisaccharide, I guess the same would apply if someone has experienced it working with peptide epitopes. I am wondering if binding specificity can change...

03 December 2019 7,361 3 View

In ICC, are there any negative outcomes from combining the blocking and primary antibody incubation steps together?

Here is my thinking: In theory, antibodies have higher affinity for their antigens/epitopes than the proteins in the serum used for blocking. Why not have them compete for the binding sight at the...

06 July 2019 5,587 6 View

Does 4T1 murine breast cancer cell line express any identified tumor antigen to function as a therapeutic target for vaccine development ?

I am looking for identified MHC-I epitopes in 4T1 murine breast cancer cell line that can be used in a therapeutic vaccine, couldn't find something useful in literature so far ! Any known papers...

06 July 2019 5,353 2 View