I am looking for cells most similar to, cholinergic + nonadrenergic/noncholinergic parasympathetic effector neurons, vagal/spinal afferents (eg DRGs) and protocols to differentiate them from suitable cell lines.
Most of the cells/protocols I can find are dopaminergic CNS type neurons.
I have been looking at SH-SY5Y, IMR-32 & NT2 which I know I can get from ATCC, but have also seen some cells such as HD10.6, LA-N-5 and MIO-M1 (all human) and NSC-34 (mouse) which would seem more suitable, but don't appear to be available.
Any advice is much appreciated
Thanks
I would recommend working with primary DRGs. Many times labs that work with mice sac them without dissecting any neuronal types at all. You can get a decent amount of primary DRGs from adult mice. Perhaps asking a nearby lab that works with mice to let you scavenge the corpses may work for you if you don't have mice in your lab. Rats even better. Adults don't generate enough DRGs for biochemistry though..
Hi Peter.
We work with F11 cells. These cells are hybrids of fused rat embryonic DRGs and mouse neuroblastoma cells. Unfortunately for you not human. But they are easy to cultivate and can be easily transfected. Under low serum conditions (with or without forskolin) they develop neurite like branches. We got them from our cooperation partners, but there are some companies which have them in their portfolio.
From the cells you named above, the HD10.6 cell line seems to be very promising. But I also couldn´t find any company to get them from. The other cell lines seem to be of other origin.
LA-N-5: neuroblastoma cells (bone marrow)
MIO-M1: Müller stem cells (retina)
NSC-34: motor neurons from embryonic mouse spinal cord cells (CNS)
Hope I could help.
Thanks for your responses both of you.
I'm specifically looking for human cells but I may have to rely on mouse/rat in the short term.
DRG from rat is good model and it will give you the bases for native neurons. rat neonatal 4-6 day you can isolate lot of DRG neurons.
Did you ever find the HD10.6? I also just got excited by finding the '99 paper that made them, then can't find them :(
Yes I tracked them down to a company in California, but I haven't got hold of any yet. Apparently they are very tricky to culture too.
Ah,"Signal Pharmaceuticals Incorporated" bought our by Celgene in 2000. And no further papers by "Raymon HK". Saw DW on the 1999 paper is still around and at Alnylam Pharmaceuticals, Cambridge, Massachusetts, USA but cant find their contact info. I think I've hit a dead end.
Hi guys. Have you found the HD10.6 cell line. I have the similar problem: Found a 1999 paper use this cells for sensory neuron research., but can't find them.
I have been in contact with Celgene, but I still haven't got any cells. For people who are looking to develop an alternative, I have been working with iPSCs according to a protocol from Chambers et al Nat Biotechnol .2013 January 01. 30(7): 715–720. doi:10.1038/nbt.2249. It seems to work quite well. See also Young et al Mol Therapy 2014.
You can culture iPSCs on vitronectin with FGF2/TGFb and adding 10mM ROCK inhibitor (Y27632) at passage. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3084903/
It's not a cheap process though especially for the SU5402.
Hello - just wondering if anyone has got hold of the HD10.6 cells or has any idea where I could buy some please?
- Badges
- Science topic
- Similar topics
- Biological Science
- Molecular Cell Biology
- Culture Cells
- Cell Line