I think the HeLa genome is stored in DBGap, and can be downloaded. You can then make your own BLAST database from the FASTA sequence.  

As I understand, the issue is that human cell line sequences are not laboratory artefacts, but the genomes of identifiable human beings. This brings special ethical concerns in releasing the data, so it is necessary to enter into a formal agreement before the data can be accessed.

thanks, i know the ethical issues of  the hela genome sequence. I tought, it was possible to query the genome for a specifc sequence.

Hi,Didier,If you just want to check a mutation of your interested gene,maybe you can try this linkhttp://cancer.sanger.ac.uk/cell_lines/sample/overview?id=1298134 and click the mutation tag.I hope you can find the information you need.

thanks...indeed may current trouble is: i am using a pair of primers to genotype a crisp expt in hela cells ..the primers are working nicely when I am using 293T genomic DNA ...the same primer do not work in PCR assay using hela kyoto genomic DNA. therefore i am wondering if the corresponding complementary sequnece are conserved/mutated in hela genome. thanks arrhenius for suggesting the sanger ressource..but it does not list my gene!

this is why I wanted to query the genome by blast. I remeber the authors of the hela genome point out that some siRNA does not works in hela just because the sequnece is mutated.

thanks ned for pointing out the NIH policy about hela genome. I am aware of this  and do not want to go into all the paper work to get access to the dgap datavbases.

i will just use others primers