Does anyone know of an antibody that can distinguish between a protein in a monomeric vs a dimeric state? This question is in regards to a homodimer (or homomultimer) complex.
Any examples would be very helpful - thank you!
Hi William M McFadden . Is there a native non-structural protein that functions both as a monomer and a homodimer/multimer?
If an antibody exists for such a creature, it must be conformationally specific.
Hi William, what's your purpose of knowing whether the protein is a monomer or dimer? Could you describe more about your upstream and downstream steps. If your dimer is formed through the covalent bond, you can simple run a SDS-PAGE to see the molecular weight. There is another way is to do a sandwich ELISA, using a monoclonal antibody as the capture antibody and the biotinylated same monoclonal as the detection antibody. Later, add the streptavidin-HRP. You see, likely, in the sandwich ELISA, the monomer cannot be recognized since there is just one epitope (if unique enough).
I think the premise is that there is a significant difference in the conformational epitopes between the monomer and the dimer of the protein you are interested in.
- Badges
- Science topic
- Similar topics
- Biological Science
- Immunology
- Antibodies