I would like to analyse some serum samples for presence of antibodies specific for the different coronaviruses SARS, MERS and SARS-CoV-2. Given the potential for cross-reactivity between antibodies against these different coronaviruses, this is a challenge. Did anyone establish serological assays that allow accurate distinction between antibodies that were induced in response to SARS, MERS or SARS-CoV-2 infection? Which antigens are most specific for each of these viruses.
Willem van de Veen
You will determine the specific antibodies in your serological tests , when you use spike protein S as antigen of the respective corona virus . It is available in some labs.
However, if you use N protein , it will get you the cross reactivity in large. I hope it'll help !!
Please also read the following link for the clarification:
Article Serological differentiation between COVID-19 and SARS infections
We use a Pseudotype based antibody neutralisation assay. We have pseudotypes made with the Spikes from CoV 2, SARS1 and MERS. We have no cross-reactivity unless of course we are testing S2 mAbs.
I would be happy to collaborate with you. My email is [email protected]
Dear Willem,
Hope this SARS-CoV-2 Neutralization Antibody Detection Kit can meet your needs.
https://www.genscript.com/cpass-sars-cov-2-neutralization-antibody-detection-Kit.html
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