I am making stable cell lines using lentiviruses. The viral particles are capable of one round of infection, so at what point is the virus no longer a safety concern? After I change the media the first time? After antibiotic selection? After a set period of time?

I will be performing functional assays with these cells using plate readers, cytometers, etc. Will I always have to ensure BSL-2 precautions with these cells (i.e. secondary storage containers, all experiments performed in biosafety cabinet, bleaching pipette tips)?