Are RNase enzyme will be reactivate in the DEPC-treated water after autoclaving and DEPC inactivation?

29 June 2022 0 457 Report

I need RNase-free water to make an electroporation solution, however, I want to autoclave the solution for sterilization. Do you think the solution will contain the enzyme again after the autoclave? Due to the fact that DEPC will be deactivated by autoclave.

I will be happy if I have your comments

Thanks

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