Antibody amino acid sequence database to simulation?

Hello, I need your help. I am looking for a database of antibody amino acid sequences for later simulation in a program in order to analyze their structure. The antibodies I need to test...

18 February 2021 371 4 View

How long can we consider that the data obtained with qualitative social research methods are "current"?

I cannot find any reference that deals with the temporal validity of qualitative data. I think there is a gap in the methodological literature on the temporal validity of the research results and,...

05 February 2021 9,788 20 View

Does DNA A-tailing survive after agarose gel electrophoresis and purification?

Hi everyone, I need to clone in pGEM-T vector the result of amplifying a metagenomic library by PCR with a high fidelity polymerase but I only want to clone the fragments of an specific size...

04 February 2021 6,051 8 View

How can I solve this problem with agarose gel DNA purification of bigger (1-4kb) fragments ?

Hi everyone, I perform a PCR + Restriction Digestion (single cut, same at both sides) over a metagenomic library. After that, I am trying to purifiy DNA fragments between 1-4kbp long from the...

24 January 2021 2,190 5 View

What is the most adequate (serum) dilution to use to determine IgE in mice serum via ELISA assay (commercial kit)?

We have a commercial kit from RayBioTech to determine IgE from mice serum. In accordance with the supplier's instructions, we have to use a dilution of 1:10000 of the serum. Is this dilution the...

06 January 2021 4,217 3 View

Is there any reference levels of IgE in healthy mice serum, like there are for other biochemical processes/components?

My question goes for reference levels of IgE in mice serum. I know that there are tables for the most common biochemical processes/compounds (red blood cells, white blood cells, AST, ALT, etc),...

06 January 2021 8,215 3 View

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It is important for me to know the opinion from your point of view as researchers.

30 December 2020 988 2 View

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13 December 2020 3,861 3 View

How can I do the bang's blinding index and james blinding index?

Hi! I would like to know with which statistical software the bang blinding index and the James blinding index and how to do it. Thanks

09 December 2020 1,363 2 View

Do anyone know a lab in Mexico where dielectric properties of solids and liquid can be measured?

I mean those kits that can be connected to vector network analyzers in order to find out permittivity and permeabilitty of solid and liquid from around 1 to 10 or 30 GHz

25 November 2020 852 1 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Can linear DNA be used as template for site-directed mutagenesis?

Is it possible to induce site-directed substitution mutation by quick-change method on linear dsDNA? or it has to be cloned in some vector? If yes, should it be treated with the Dpn1 enzyme...

03 March 2021 401 4 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

PUC19 as a transfer plasmid for lentiviral delivery?

Hello, Is it possible to use pUC19 as a transfer vector to be packed in using the second generation viral particles packaging system( pMD2.G; psPAX2 plasmids)? As far as I understand it there is...

28 February 2021 4,868 2 View

Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View

Can thermal cycler machines be problematic during PCR?

Hi, my question is about the heating of thermal cycler machines and I hope some of you had experienced a similar thing previously. There are two thermal cycler machines in the lab(BioRad) and for...

26 February 2021 4,777 4 View