Are Platinum™ Taq DNA Polymerase High Fidelity (invitrogen) compatible with pGEM easy vector ligation?

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How to Input and Analyze Results for an Asymmetrical Factorial Design in STATISTICA 13.3?

In STATISTICA 13.3, I used the Experimental Design Builder under the Design of Experiments tab to generate a design matrix for an asymmetrical factorial design (1443) with 12 experiments. However,...

29 July 2024 4,155 2 View

What is the relationship between Artificial Intelligence and Communications?

Much is said about AI and the Communication field. Some say that Communications would very much benefit from that intersection. But specifically which areas would benefit from AI? Could it be a...

11 July 2024 5,335 4 View

Protein degradation? how to concentrate low MW proteins?

Hi everyone! So I am trying to concentrate my recombinant proteins in order to make some assays with them, but I need a "considerable" concentration of each. One of my proteins is around 9kDa and...

08 May 2024 5,418 2 View

How reliable is Fiji software for analysis of movement organisms?

Dear all, My question is addresses to reliability regards how reviewers or journal editors would take it when they realize that the methodology of a paper was mainly done through this software. I...

15 April 2024 9,305 0 View

What is the refractive index of MoSi2?

Does anyone know what is the refractive index of MoSi2 and in what medium it can be performed (distilled water or ethyl alcohol)? I pretend to perform a particle-size distribution analysis by...

23 March 2024 6,736 1 View

Where to order research-grade Galleria mellonella?

Does anyone know where to purchase research-grade Galleria mellonella in Europe? I only find the TruLarv from BioSystems Technology, but it seems to be down... Thank you!

11 March 2024 4,800 2 View

Could someone recommend me a valid instrument to measure teaching performance that utilizes AI (Preferably focused on elementary school teachers)?

I am conducting research on the performance of primary school teachers who use AI, and I have not been able to find a validated instrument that fits this situation. I would like to hear your...

22 February 2024 6,052 4 View

Sauropod-like vertebrate teeth?

Is is possible that Cretaceous fish, notosuchians, lepidosaurs, or other vertebrates have a morphology that might be confused with "spoon-shaped" sauropod teeth? I have seen a fossil isolated...

08 December 2023 7,040 2 View

Could someone provide me with two JCPDS cards?

I would need two JCPDS cards: 1. BaO (No. 26-0178) 2. Ba(OH)2 (No. 026-0154) I would appreciate a lot if someone could send me both cards... I am a bit desperate. Thanks again for your time!

08 October 2023 4,324 4 View

Is there an accepted taxonomy or categorization of environmental issues (e.g., waste management, water pollution, etc.)?

I am currently working on enhancing the H4rmony dataset, which aims to fine-tune Large Language Models in AI to align them with ecolinguistic values. I'm seeking this classification to assist in...

27 September 2023 972 3 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Does crude extraction using NaOH and Tris work well with Fungi?

I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you

08 August 2024 4,733 2 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

Why my colony PCR results of my recombinant bacterial not showing any results?

I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....

05 August 2024 2,570 3 View

Low-yield gel extraction problem?

I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...

05 August 2024 9,798 3 View

How to load and plot 2D-PIV (particle image velocimetry) recorded velocity vector field in Techplot?

Dear Researchers I need to know, how to load and plot 2D-PIV (particle image velocimetry) recorded velocity vector field data in Tecplot? Thank You

02 August 2024 3,615 1 View

Does anyone have issues using Prepman Ultra reagent for MicroSeq ID bacterial, fungal and yeast sample preparation?

I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...

01 August 2024 2,079 0 View

Transfection in HEK293T cells?

Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...

31 July 2024 9,892 4 View