Are Myxomycetes species with dark spores truly bright spores in phylogenetic clade?

More Leandro Almeida Neves Nepomuceno Agra's questions See All

What are your tips to write the discussion section of your scientific work in an efficient way?

Writing the discussion section is always the scariest part of a paper or a thesis. I came up with some ideas to make it less difficult, but would like to hear other tips! Here are my two bits:...

04 November 2020 6,808 3 View

How can I determine the total antibodies in the serum of bovines with ELISA?

I want to determine if there is a difference in the amount of total antibodies in the serum of different bovines, I was thinking of doing it with Elisa sandwich. Has anyone tried it? What initial...

13 August 2020 2,412 4 View

Can I performe behavioral tests in the same rats at PND30 and PND60?

Hi! Anyone knows whether I can performe behavioral tests in rats at PND30 and then, repeate them at PND60? Or this is not correct? Thanks.

20 July 2020 3,659 3 View

Where can I find an available multimodal biometric dataset?

This dataset will be used in the context of a University Course.

27 June 2020 3,958 2 View

What measures have your labs/ institutions put in place to mitigate the impact of COVID-19 on early career-researchers?

Early career-researchers are disproportionately affected by the COVID-19 pandemic in academics, as they often struggle with precarious working situations and they are seeing many doors shutting...

27 May 2020 1,440 1 View

Avigan ( Favipiravir), Hydroxychloroquine plus Azithromycin. A possible Cocktail against SARS-COV2 ?

All the three medicaments have promising results reported and supposed complementary mechanism of action?

10 April 2020 6,909 7 View

Simulation Parameters for p-type and n-type layers of a typical silicon soalr cell?

Hi, I would like to simulate a typical polycristalline silicon cell with p-type and n-type layers using a drift diffusion model. I have found a lot of references for intrinsic silicon parameter...

03 February 2020 3,203 6 View

In Limma package, why should I linearize my data in some analyses?

tl;dr: Why data linearization is applied in the identification o differentially expressed genes/proteins? I am new to data analysis of big data like proteomics. As far as I know, a simple t-test...

16 November 2019 8,441 4 View

Does anyone know of a practical energy efficiency model for wireless sensor networks (WSN) or software that uses attributes of that model?

I have already found some reference authors who model some parts of the system, for example, sensor, transceivers, link, propagation space, the network itself and, sometimes, putting it all...

11 November 2019 2,111 3 View

What is the minimum level of energy that allows a near body device to be long term self-sustainable?

My concern is about Energy Efficiency Model for WBANs. In a wide sense, I'm looking for ways to model the whole network in terms of power and energy. How much average power is required to keep...

11 November 2019 5,053 4 View

Isolates of Hymenoscyphus fraxineus?

Dear Dr. Cai, my name is Simone Prospero and I work in the team of Phytopathology at the Swiss Federal Institute for Forest Snow and Landscape Research (WSL;...

01 March 2021 1,133 1 View

How to remove the residual PDMS on the silicon mold?

Incompletely cross-linked pdms are difficult to remove.

01 March 2021 798 3 View

The reason that the culture of white fungi Contamination with green mold(Trichoderma), the culture autoclave at121 for 15 min. What is it?

Is the period to autoclave not enough? The inoculation in a hood and flame and UV and alcohol.

27 February 2021 9,356 3 View

What is the most suitable medium of ascomycetes fungi ??

26 February 2021 4,413 1 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Can 5-FOA Diffuse Through Agar?

I am preparing to do a gene knockout in the filamentous fungi Coccidioides posadasii targeting pyrG/URA3 so that 5-FOA can be used for selection pressure instead of traditional antibiotic...

16 February 2021 169 4 View

Does the phylogenetic tree making tool IQTREE use evolutionary placement algorithm?

For my master thesis I am conducting a phylogenetic research and for this I'm using tools like MEGA, IQTREE, FigTree, etc to make a phylogenetic tree. I wanted to know if IQTREE integrates the...

14 February 2021 1,541 2 View

Can one use just 250 micron sieve & still get a good number of mycorrhizal spores from the soil?

In a situation where one cannot get the appropriate & separate levels of sieve for mycorrhizal survey work, can just one level of sieve give good results & spore counts?

13 February 2021 3,760 2 View

Any specific reason, how sclerotium rolfsii can grow out of Petridish?

Is it related to nutrition or any other reason? Any other fungus have similar kind of growth pattern?

09 February 2021 4,357 4 View

Which fungicide are good for primary cells? Does it yeast contamination? How to get rid of them without killing the cells? How to get rid of spores?

Hello! Our lab is working with primary cells which we are isolating from the colorectal tissue. During the isolation, we are washing the tissue with pen-strep and then also add some pen-strep in...

07 February 2021 8,258 3 View