Why doesn't my RNA loading look uniform?

I am running formaldehyde-agarose gel electrophoresis to perform non-radioactive northern blotting. I know northern blotting is a very old story now, however, I am trying to study different...

21 September 2020 8,153 4 View

How to design "A Tunable RF Front-End With Narrowband Antennas for Mobile Devices" ?

I don't know how to design the architecture using the PCB board and how to test the fabricated prototype ? can you help me out of this issue?

17 December 2019 9,068 4 View

Between Julia and MATLAB, which would be the most efficient for a discontinuous Galerkin FEM code?

I need to simulate a 2-D problem with DGFEM. I have been advised to use C/C++ for a code that delivers quick and efficient results, however, I am not very comfortable with them, and my base code...

16 December 2019 7,001 5 View

Can total energy Vs k-mesh increase to higher values in DFT-VASP?

I am performing DFT calculations on halide perovskites using VASP code. I began my calculations on CsPbI3 where I took 6.2 Angstrom as lattice parameter and built the cubic structure in VESTA....

16 September 2018 9,316 6 View

Can you please suggest a good bioinformatics textbook/guide for gaining expertise on multiple sequence alignments?

Hello all, I am looking for recommendations on a good bioinformatics textbook/guide which helps me gain sound background knowledge on multiple sequence alignments. A part of my research is to...

31 October 2017 1,582 3 View

96 well plate procedure for TPC and TFC?

31 January 2017 2,927 4 View

How can I minimize the air bubbles during a western blot wet transfer?

Hello everyone, I am right now facing the issue of air bubbles during the western blot wet transfer method. It is so frustrating that some silly air bubbles mess up the whole results. This is what...

20 March 2015 6,082 27 View

How can I induce DNA damage using Temozolomide?

Hi all, I am currently studying activation of PARP after inducing DNA damage. The method I follow is: 1) exposing tumor cells (HCT116, HeLa cells) and MEFs (WT and S12) to DNA damaging agent...

01 March 2015 3,311 3 View

Can anyone explain to me about tagged western blotting?

I am curious about the tagged western blotting, where, proteins are probed on a nitrocellulose/PVDF membrane with HIS-tag/GST-tag primary antibody initially, and later with a corresponding...

01 March 2015 6,492 3 View

What is the BRCA status of the colon cancer cell line HCT116?

I am trying to put together the BRCA and p53 status of various cell lines like HeLa, WT/S12 MEFs, HCC1937, MDAM231, MCF7, HCT116. I was able to get the statuses of every other cell line, but just...

30 November 2014 2,838 3 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

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02 March 2021 3,060 3 View

Where can I find life cycle inventory or assessment of non woven materials?

Dear network, I am looking for the LCA or LCI or at least the energy balances of the non woven fabrics process for my master project, in specific for natural fibers, yet I have found...

02 March 2021 9,662 1 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View