15 Questions 13 Answers 0 Followers
Questions related from Shruthi Gobbooru
Hello, I am trying to study the preferential partitioning of an RNA containing long 3'UTR into stress granules (SG), as compared to an RNA isoform with shorter 3'UTR. A method that I found in...
05 July 2022 5,121 0 View
Hi, I am studying the selective partitioning of poorly translating RNAs with long 3'UTR into stress granules. I am using Thapsigargin to induce ER stress. Does anyone know how soon can I start...
14 June 2022 7,707 0 View
I am running formaldehyde-agarose gel electrophoresis to perform non-radioactive northern blotting. I know northern blotting is a very old story now, however, I am trying to study different...
22 September 2020 8,229 4 View
I am planning to study global protein synthesis levels in presence and absence of certain chemical compounds. I know that the gold standard for this purpose is the S35-methionine pulse chase...
27 September 2019 4,323 2 View
We don't have a Stratalinker or any UV crosslinkers in our lab or our department. Is is absolutely necessary to crosslink RNA while doing a Northern blot? I am planning to do a nonisotopic...
18 September 2019 3,546 2 View
Hi, I need to look at the RNA of my gene of interest. I am planning to do non-radioactive northern blotting for this. Does anybody know of a protocol to synthesize biotinylated cDNA probe using my...
03 June 2019 2,446 1 View
Hi all, I am working on dual luciferase assays using Dual-Glo kit (Promega). My experimental vector is pMIR-report (with my inserts cloned into the MCS) and control vector is pRL-TK. The ratio of...
17 August 2018 8,685 11 View
Hi all, I am trying to study the effect of oxidative stress on my reporter gene activity, in hek293 and hela cells. I searched a lot to see if anyone did something similar, but couldn't find...
14 August 2018 2,804 2 View
I am trying to study cell cycle arrest in mammalian cells and wanted to use methotrexate. I obtained the IC50 value for methotrexate in HeLa cells but couldn't find for HEK293 cells. Rather, there...
31 July 2018 844 1 View
Hello all, I am looking for recommendations on a good bioinformatics textbook/guide which helps me gain sound background knowledge on multiple sequence alignments. A part of my research is to...
01 November 2017 1,638 3 View
Hello everyone, I am right now facing the issue of air bubbles during the western blot wet transfer method. It is so frustrating that some silly air bubbles mess up the whole results. This is what...
21 March 2015 6,144 27 View
Hi all, I am currently studying activation of PARP after inducing DNA damage. The method I follow is: 1) exposing tumor cells (HCT116, HeLa cells) and MEFs (WT and S12) to DNA damaging agent...
02 March 2015 3,363 3 View
I am curious about the tagged western blotting, where, proteins are probed on a nitrocellulose/PVDF membrane with HIS-tag/GST-tag primary antibody initially, and later with a corresponding...
02 March 2015 6,560 3 View
I am trying to put together the BRCA and p53 status of various cell lines like HeLa, WT/S12 MEFs, HCC1937, MDAM231, MCF7, HCT116. I was able to get the statuses of every other cell line, but just...
01 December 2014 2,911 3 View
Hi all ! I am currently in the second trimester of pregnancy. I, however, have some research deadlines and it means I have to consider working with chemicals I initially decided not to during...
01 January 1970 3,041 1 View
