How can you attach polyurethane devices on glass slides?

I am making microfluidic devices made of polyurethane. The problem I am facing is that I am unable to attach these devices to glass slide even after plasma treatment (air plasma). Some of the...

28 January 2021 5,194 2 View

Is the assumption about Delaunay edge true?

01 July 2020 9,998 2 View

Lysis Buffer for RNA extraction?

Dear all, I am working on a magnetic beads based RNA extraction project. Presently, I managed to come up with a lysis to extract RNA from blood samples. However, my PCR results show inhibitory...

06 April 2020 9,953 3 View

Homing Receptors for tumor infiltrating T cells?

I am researching how different culture and expansion conditions contribute to a T cell's infiltration of a solid tumor. Was wondering what the best surface / intracellular receptors or ligands are...

26 June 2019 5,072 0 View

What is the source of basic 'hydroxyl' sites on gamma-alumina surface? Can hydroxyl ions form on its surface with only water present?

Consider an initially non-hydrated gamma-alumina surface. I pass water over the surface hoping it to dissociatively adsorb on the surface giving me hydroxyl radicals adsorbed on alumina surface....

31 March 2019 8,615 2 View

Does any one have a detailed protocol for isolation of Glucocerebrosidase from rat brain, please don't mind to share?

I am working on Gaucher"s disease, in this connection I would like to isolate GCase enzyme from rat brain. so please share the protocol if available. This isolation process should not include HPLC.

27 March 2019 2,591 1 View

Can anyone help me with my Horn Antenna project for 21 cm astronomy?

Me and a group of first year undergrads from my college are working on building a horn-antenna for radio astronomy similar to this...

16 April 2018 8,040 3 View

Can anyone suggest best possible resin for the Human Beta-2-Microglobulin purification from an albumin (60% - 80%) containing sample?

I have a feed containing B2M, albumin and antibody fragments from which i want to purify B2M as my sole target protein using Chromatography resin in one or two steps. If anyone has any good...

12 December 2017 9,312 3 View

Orthogonality of Asymptotic/WKB solution

It is known that the exact eigen-functions of sturm-liouville problem are orthogonal with respect to the weight function. But what happens when the solutions are obtained using approximate...

06 June 2017 486 3 View

How to store geosmin, a highly volatile compound, after breaking the glass ampule?

I have purchased a highly volatile compound known as geosmin. It was contained in a glass ampule. Geosmin was dissolved in dichloromethane (DCM). I made several aliquots of 30Ul in a 1.5ml...

17 January 2017 5,647 3 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

How to calculate Total Flavonoid?

I analyzed flavonoid total spectrophotometry, the procedure as below: 2 ml supernatan added 5 ml dd.H2O, then added 0,15 ml NaNO2 5%, 5 minutes later added 0,15 ml AlCl3 10%, After 6 minutes,...

01 March 2021 6,722 1 View

RNA-seq DEGs downstream analysis and reporting in literature?

I used EdgeR to get the list of DEGs (differentially expressed genes) from my liver RNA-seq experiment. I have done the gene overrepresentation and GSEA based pathway analysis. I am currently...

23 February 2021 8,969 1 View

What are the ranges of four diffterent poles of butterworth filter for ECG signal?

Hi, Can anyone please tell me what are the ranges of four different pole orders such as first order, second order, third order and fourth order respectively of butterworth filter for ECG...

23 February 2021 2,554 3 View

Which polarizers are usually used to measure the birefringence of zebrafish muscles?

I already found several publications on that topic, however, the specifications for the used filters are often missing. Some more specific information than "fish is placed between to polarizing...

23 February 2021 1,934 1 View

What low-cut filter (high Pass frequency) to be used for response spectrum (Tripartite plots) ?

What low-cut filter (high Pass frequency) to be used for the response spectrum (Tripartite plots)? I am currently working on differentiating between hard/soft site responses in Indo-Gangetic...

21 February 2021 9,273 2 View

Is it possible, for a limited dateset of images, to use Convolution filters + Random Forest instead of UNet?

UNet is 'relatively' recent and was invented mainly to deal with biomedical images, yet is it also possible, for a limited dateset of images, to use Convolution filters + Random Forest instead of...

21 February 2021 4,858 3 View

The type of filter paper used to filter an epoxidized oil (the mixture is an epoxidized oil with magnesium sulfate) ?

I have a mixture of epoxidized oil and magnesium sulfate wich type of filtre paper should i use

21 February 2021 3,944 3 View

What is difference between In-line transmission (%) and Total transmission (%) value of optically transparent Ceramics?

I am working on transparent/translucent zirconia-based ceramics. In many papers, I have seen that they are reporting both In-line transmission (%) & Total transmission (%) value of...

20 February 2021 9,405 6 View

Algorithms to filter corrective max and min from a set of data?

I've got this 5 year Direct Normal Irradiance data I downloaded from https://re.jrc.ec.europa.eu/. I ordered this by year and by every 24 hours because I need the DNI curve by max in summer, min...

17 February 2021 2,777 5 View