I would like to measure dopamine receptor (protein) expression in human blood plasma. Is it possible to use ELISA for this?
Dear all, I'm going to radiolabel antibodies. So first of all, I'll remove excipients using the centrifugation method with amicon. Once I'm done, how do I calculate/measure the concentration of...
14 July 2024 3,106 3 View
Dear researchers, I would like to radiolabel pembrolizumab with zirconium-89 but first I need to remove excipients by ultrafiltration or concentration. I'm thinking of doing it using...
24 April 2024 8,163 0 View
Dear all, I would like to radiolabel pembrolizumab with a metal and would like to remove the excipient. I read in some paper that I can use centrifugation but have no idea of how it is supposed to...
05 February 2024 5,065 0 View
Dear Researchers, I'll be working on conjugaison reactions with monoclonal antibodies, have somebody already done that before ? This is my first time using monoclonal antibodies ? What about...
22 June 2023 6,624 3 View
Hi everyone, I'll work on zirconium-89 radiolabeled antibodies and would like to have some help for conjugate antibodies purification. I'm thinking about using PD10 column, is it a good idea ?
12 April 2023 5,302 6 View
Dears collegues, I obtained strange derivative curves (difference between samples) of my PCR HRM : the peacks are angular. It's a 150bp amplification of ADN from a bacterial communinities with...
06 April 2023 2,304 0 View
Hello everyone, Does anybody have experience with electroporation of Cholangiocarcinoma cell lines? I was trying to find published settings for a pilot experiment involving the electroporation of...
13 March 2023 1,953 0 View
Hello all, I've been trying to stain tiny organs with eosin yellowish acid 87 in ethanol 70% before paraffin embedding but the staining does not last after the dehydration process. I've read some...
15 July 2021 5,952 5 View
Hello, I am interested in the personalization of learning based on profiles, more specifically in mathematics. Do you know any relevant references? Thank you
11 October 2020 4,691 0 View
Hi everyone, If somebody can explain to me what's happening to my samples it would be great ! I'm currently testing a RT-qPCR for three primers, one of wich can amplificate genomic DNA if...
23 August 2020 5,145 3 View
I would like to learn more about SPSS and Its application especially in regards to data analysis. Please suggest me how I can learn more about it. Thank you so much.
11 August 2024 9,101 4 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
Hello, Why do i see this baseline drift when i compare my blank (black) to the sample (blue)? Any suggestions as to why this happened? Thank you!
11 August 2024 3,770 4 View
Willett, Shenoy et al. (2021) have developed a brain computer interface (BCI) that used neural signal collected from the hand area of the motor cortex (area M1) of a paralyzed patient. The...
10 August 2024 7,180 0 View
I'm currently exploring the application of Python in textile engineering, specifically in areas like data analysis, process automation, and the development of smart textiles. I'm interested in...
10 August 2024 7,429 2 View
How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?
09 August 2024 7,718 0 View
Let's say we have a standard, regular hexagonal honeycomb with a 3-arm primitive unit cell (something like the figure attached; the figure is only representative and not drawn to scale). The...
07 August 2024 1,937 1 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
A fungal strain was treated with nanoparticles. We want to do an environmental SEM analysis. So could anyone share your views on preparing the sample? Thank you.
07 August 2024 5,307 1 View
I have been getting a low plasma and no coating while doing rf sputtering of copper doped ZnS using a power of 140 watts and at pressure of 6.5x10-3 Mb.
07 August 2024 4,526 4 View