Hey Filipe,

I think the regular lipofectamine based methods work according to the literature search.

http://www.lifetechnologies.com/us/en/home/life-science/cell-culture/transfection/transfection-support/transfection-selection-tool.html

Any how, check this paper too.

http://www.ncbi.nlm.nih.gov/pubmed/19360711

All the best for the experiments.

Hi Filipe,

we regularly transfect C2C12 with Lipofectamine 2000 for plasmids, or Lipofectamine RNAimax for siRNA. It works perfectly.

Good luck!

Hi Filipe,

I would definitively recommend an alternative: DreamFect Gold. It is indeed a lipid-based transfection reagent, but highly efficient and biodegradable (high viability).

you can refer to : http://www.ncbi.nlm.nih.gov/pubmed/21248078 for more details,

do not hesitate to contact me at [email protected] if you need any information

Cedric

C2C12 cells are undifferentiated cells so any commercial mammalian transfection kit should work. These are relatively easy to transfect compared to hard to transfect cells like macrophages for which we use specific transfection kits. Lipofectamine, genePORTER all will work. 

Vivek 

Hi Filipe!

I agree with the usage of lipofectants like lipofectamine 2000. Back some years ago, we also used precipitation with calcium/phosphate with fair performance. Good luck!

Hi Filipe:

Can you tell me how did you make the transfection, in undifferentiated or differentiated C2C12 cells?

Hi Valeria,

In the end we used Lipofectamine2000.

Best