Valeria Viteri Alvarez

4 Questions 6 Answers 0 Followers

Questions related from Valeria Viteri Alvarez

Anti-human CD4 antibody (IHC)?

Does someone have experience with an anti-human CD4 antibody that works well in IHC (paraffin)?

14 October 2021 7,188 1 View

How to culture human MDSCs for 48 hours before using them for the experiments?

Since I want to perform experiments at different time points, I would like to culture MDSCs so that they can be used after 48 hours of being isolated from the patients' blood. I want to be able to...

14 September 2020 2,088 2 View

Does anyone have experience with fMLP as a neutrophil chemoattractant in a transmigration assay?

I am planning to perform a transmigration assay (transwell plate) in which are involved neutrophils , therefore I will use fMLP as a chemoattractant. I did find some papers that describe "The...

20 February 2020 5,967 3 View

In wich time-points its recomendable to measure ROS in a I/R in Vitro Model (C2C12 cells) after the reperfusion ?

Im doing a I/R model with C2C12 cells and I need to measure ROS production. First I will induce ischemia with the ischemia chamber and then I will measure ROS after 4,6, 8 and 12 hours. In the...

27 June 2017 3,914 2 View