Valeria Viteri Alvarez

5 Questions 7 Answers 0 Followers

Questions related from Valeria Viteri Alvarez

Anti-human CD4 antibody (IHC)?

Does someone have experience with an anti-human CD4 antibody that works well in IHC (paraffin)?

14 October 2021 7,237 1 View

Does someone have experience with T cell exhaustion using positively isolated CD8+ T cells (human)?

For my project, I need to use CD8+ T cells and follow exhaustion protocol. There are usually kits available with negative selection to obtain untouched CD8+ T cells. Due to the way my experiment...

12 March 2021 4,922 1 View

How to culture human MDSCs for 48 hours before using them for the experiments?

Since I want to perform experiments at different time points, I would like to culture MDSCs so that they can be used after 48 hours of being isolated from the patients' blood. I want to be able to...

14 September 2020 2,134 2 View

Does anyone have experience with fMLP as a neutrophil chemoattractant in a transmigration assay?

I am planning to perform a transmigration assay (transwell plate) in which are involved neutrophils , therefore I will use fMLP as a chemoattractant. I did find some papers that describe "The...

20 February 2020 6,032 3 View

In wich time-points its recomendable to measure ROS in a I/R in Vitro Model (C2C12 cells) after the reperfusion ?

Im doing a I/R model with C2C12 cells and I need to measure ROS production. First I will induce ischemia with the ischemia chamber and then I will measure ROS after 4,6, 8 and 12 hours. In the...

27 June 2017 3,951 2 View