I`m trying to prepare a few samples and I found that some of the vitamin B compounds (e.g. Riboflavin and folic acid) are not very soluble in water. Can they, and others, be soluble in DMSO?
DMSO is the used for all new compounds screening in drug discovery. Therefore, it is your solvent of choice. It has always a few percentage of water that helps for less hydrophobic compounds.
Folic acid and Riboflavin does dissolves in DMSO at low concentrations, from personal experience. Undisturbed solutions will show some precipitation after a day at high concentrations, say, 10 mg/ml.
However, you should be able to dissolve the folic acid to about 0.5 mg/ml in a 1:1 mix of DMSO:PBS buffer (pH ~ 7.4). I would suggest, please try mixing with DMSO first and then add the buffer. Cheers!
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