Apart from western blot, what are the other tests that can be used to determine expression of autophagy-related proteins ?

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Shiqiu Xiong

Hi Santha:

You can also try immunofluorescence, test subcellular distribution by confocal, or assess expression amount by flow cytometry; the other options are: mass spectrometry; qPCR et al if the facility is available.

Best regards

Shiqiu

Kavita Rawat

You can perform, fluorescence microscopy, immunofluorescence microscopy, flow cytometry and TEM.

Tariq Khan

ELISA for quantitative expression