In situ hybridization on cre-recombinased mice protocol?

Has anyone done FISH on cre-recombinased mice? I want to know if anyone has successfully been able to quench the autofluorescence from the those mice to successfully visualize the targeted genes?...

31 December 2019 5,028 3 View

Liquid chromatography mass spectrometry (LC-MS) and condition media analysis?

Has anyone done LCMS on condition media for protein quantification? I have this media (after the exposure of different flow conditions) that i would like to use LCMS for protein quantification...

01 February 2018 1,344 8 View

How can I make potassium free DMEM media?

Any ideas on how to make a potassium free DMEM media or any company that already sells potassium free media without customizing it? I have reached out to few companies and their rates are super...

31 December 2017 1,419 2 View

Porcine vascular endothelial cell isolation - from the aorta?

Hello, Are there any protocols for the isolation and purification of porcine vascular endothelial cells (from the aorta)? I would like to have my study from the primary culture instead of...

09 October 2017 9,578 3 View

Condition media assays?

Hello, I was wondering if anyone knows different conditioned media assays to quantify protein concentrations besides using ELISA. I have conditioned media from arias treatments and I want to do...

08 September 2017 3,674 2 View

RT-qPCR data analysis..(deltadeltaCt method)?

Hello, I have three different groups (Static/A/B) and I want to use delta delta Ct method to show my results in a log form. Previously I always had an untreated control to do the delta delta...

01 February 2017 7,676 3 View

Any good ALPL primer set for porcine?

01 February 2017 386 1 View

How to perform cDNA and primer quality assessments?

Hi, I have recently been trying to run RT-qPCR experiments for interstitial cells. I used Qiagen RNA extraction kit where I got ~500ng/ul of RNA with 260/280~2 and 260/230~1.9, which shows me that...

31 December 2016 7,986 12 View

How to avoid interstitial cell contamination from endothelial cells?

Does anyone know of a method/tip to avoid interstitial cell contamination from endothelial cells? I have been trying to isolate valvular endothelial cells (pig) and keep getting interstitial cell...

11 December 2016 8,311 4 View

Where to start learning molecular biology/signaling pathways?

I want to start learning about different signaling pathways and post transcriptional pathways.. I am not sure where to start. Any help/tip/resources would be greatly appreciated

11 December 2016 4,716 2 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

1. What is the impact of having different scales in a survey? and how can we solve this problem before and after data collection (Literature)?

1. What is the impact of having different scales in a survey? and how can we solve this problem before and after data collection (Literature-based reflection)? Thank you for your time and for...

03 March 2021 2,870 3 View

What degree of missing data in a particular case/participant results in Expectation-Maximisation no longer working?

I'm dealing with a mediation model and am using the PROCESS module in SPSS. Due to SPSS and PROCESS being limited in the imputation methods - being unable to handle multiple imputation - the other...

02 March 2021 4,362 1 View

Can NFL theorem be valid in infinite search space in ML?

NFL theorem is valid for algorithms training in fixed training set. However, the general characteristic of algorithms in expanded or open dataset has not been proved yet. Could you show your...

01 March 2021 1,189 3 View

Does anyone know how to plot my station to a section plot on Ocean Data View (ODV)?

I made a vertical section plot with WOA .nc file on ODV, and now I want to plot my stations (csv. file) in it. Does anyone know how to import my points to the section? They are two different...

01 March 2021 3,610 1 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is there a method of determining feed digestibility without the need to re-pellet the feed?

I am aiming to determine the ADC values of a commercial fish feed, but I do not have a steam pelleter or extruder available to me. Ideally, the pellets could be coated with some inert marker....

28 February 2021 7,967 3 View