Dear all. I'm following a procedure in order to use AOM (Azoxymethane) in a mice colorectal cancer inducing. I've determined I need only 20 microliters from a glass container of 100 microliters of commercial AOM. Can I only use 20 microliters and storage 80 microliters in a sterile glass container? Or maybe the remaining AOM will evaporate and concentrate? What is the best choice in order to preserve the remaining 80 microliters (temperature, diluting, etc.). Thanks in advance.
Dear Liceth Cuellar
Yes it will work, Tightly closed container under an inert atmosphere, and store it in an explosion-proof freezer. STORE AWAY FROM SOURCES OF IGNITION , Sigma recommends Storage Temperature −20°C
Pls. find the attached files Handling Procedures & Storage Requirements
http://datasheets.scbt.com/sc-252398.pdf
http://www.sigmaaldrich.com/catalog/product/sigma/a5486?lang=en®ion=SY
https://pubchem.ncbi.nlm.nih.gov/compound/azoxymethane#section=Handling-and-Storage
http://www.chemicalbook.com/ProductChemicalPropertiesCB9487512_EN.htm
https://www.mcgill.ca/research/files/research/712-_use_of_azoxymethane_in_rodents__2013.pdf
https://safety.tau.ac.il/sites/safety.tau.ac.il/files/media_server/safety/SOP%20for%20Azoxymethane%20in%20animals.pdf
http://www.sigmaaldrich.com/catalog/product/sigma/a2853?lang=en®ion=SY
Certain Ammonium Octamolybdate Isomers, Inv. 337-TA-477 DIANE Publishing ISBN
Hoping this will be helpful
Regards
Prof. Houda Kawas
Yes, but you must aliquoted in small amounts (which you will use) and freezing (-20°C)
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