I would appreciate any suggestions or recommendations about articles that could help me understand how these assays are performed, and what reagents/equipment are needed to do this.
It is important to highlight that technological surveillance on oceanographic vessels is a collaborative effort in which different specialists work together to effectively leverage technology in...
31 July 2024 1,633 0 View
Due to the shifting ocean currents, the planet could be thrown off balance, altering the angle of rotation, and even causing the axis to shift, plunging us into chaos. In reality, it doesn't take...
15 July 2024 8,949 1 View
I am looking for a methodology to evaluate the quality of wastewater using zebrafish larvae and embryos. Test No. 203: Fish, Acute Toxicity Test generally describes the procedure, but I have...
16 June 2024 6,473 0 View
Wind turbines extract a lot of energy, about 30-35%, that passes through. Now they are building a lot of them, more and more powerful. Change wind trajectory is easy, if a lot of obstacles is in...
12 June 2024 9,002 2 View
Dear colleagues, I have spent a few weeks trying to figure this problem out with no success, so that I post this question here. First of all, I have to say I am new to LBM method. I am working...
11 June 2024 1,084 3 View
I'm trying to upload a recently published book to Researchgate and the response is: "An error occurred. Error parsing server response".
01 April 2024 485 2 View
I work in a investigation about hydrolysis of protein of a andean grain and is very important for continue with my work what method is more effective for the activation or maybe is possible not to...
10 March 2024 6,629 2 View
in the given regular triangle ABC find the locus of the points M inside this triangle such that MAB +MBC+MCA=pi/2
07 March 2024 7,514 5 View
In triangle ABC, the median BD is drawn from vertex B to side AC. If the length of AC is 10 cm and the length of BD is 6 cm, what is the length of the median AE drawn from vertex A to side BC?
02 March 2024 7,053 2 View
The product was previously available at Millipore Sigma, but for the past six months we have not been able to order it.
15 February 2024 2,647 1 View
I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.
07 August 2024 6,622 1 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi all! My thesis groupmates and I are working on purifying an E. coli bacteriophage. The phage was handed down to us by the previous thesis group, but they only managed to purify it to about...
06 August 2024 5,801 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
Evolutionary fitness is based on an organism’s ability to adapt rapidly to changing environmental circumstances. Large-bodied mammals have been equipped with large brains (and hence a high...
06 August 2024 4,849 2 View
Air moisture harvesting Air water collection devices
06 August 2024 5,473 2 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...
04 August 2024 8,894 1 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View
I would like to perform transfection with the reagent DharmaFECT Duo (Horizon) on the AGS cell line. Could you please inform me of the optimal concentration to use without causing cytotoxicity in...
03 August 2024 3,851 1 View