Anyone with a reliable source of cross-adsorbed antibodies?

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Does anybody produce 6xHis-tagged cutinase from Erysiphe graminis, preferably for expression in E. coli?

I am looking for plasmid or bacteria expressing 6xHis-tagged cutinase from Erysiphe graminis for purification. Thanks a lot in advance

09 October 2017 8,348 0 View

Any advice on cell permeable DNA stain compatible with plants?

Anyone has experience with a truly permeable DNA stain that will not be adsorbed in plant cell walls? We work on Arabidopsis roots and we tested dyes that are membrane permeable in animal cells...

10 November 2015 2,033 7 View

Where can I get kits for co-immunoprecipitation?

I am looking for efficient kits for Co-IP using mouse and rabbit IgGs. Effectively co-IP output should be minimally contaminated with IgG heavy/light chains. Any idea?

08 September 2014 4,823 10 View

Does anyone have information on online tools for Ramachandran plotting?

I need a link to an online tool where I can upload small peptide sequences or FASTA format proteins that do not necessarily have a PDB accession. Any idea?

06 July 2014 7,084 2 View

Which homo- or heterobifunctional crosslinkers are cell permeable and which aren't?

Homo- or heterobifunctional crosslinkers were sparsely reported in the literature as putative fixatives for immunolocalization microscopic studies. Does anybody have information on which...

03 April 2014 1,715 1 View

High pressure freezing of Arabidopsis thaliana roots.

Does anyone have experience with high pressure fixation regimes for plant tissues with different vacuolation degrees. Primarily I would like to apply HPF to Arabidopsis roots, but I would greatly...

02 March 2013 4,842 1 View

High refractive index mounting media.

Does anyone have any ideas about high refractive index mounting media for either fixed, immunolabeled samples or for living, GFP-fusion expressing cells, to avoid spherical aberrations with high...

01 February 2013 8,317 2 View

Does anyon have suggestions for aqueous two phase partitioning?

Can somebody attach a step by step and thorough protocol for aqueous two phase partitioning of Arabidopsis root plasma membranes? I am not experienced and I need an easy to follow approach.

09 October 2012 5,080 4 View

Is anyone aware of the penetration rates of aldehyde fixatives commonly used for chemical fixation?

For example formaldehyde, glutaraldehyde or acrolein? I am particularly interested to understand whether soluble proteins may be largely relocated during chemical fixation.

08 September 2012 9,186 26 View

35S promoter of cauliflower mosaic virus

I want to ask whether growth conditions of Arabidopsis seedlings could affect the efficiency of transgene expression driven by the 35S promoter of cauliflower mosaic virus. I am having problems...

08 September 2012 9,967 26 View

Mouse CD3 antibody sequence?

Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much

08 August 2024 9,157 4 View

Can I use a HisTRAP column for affinity chromatography?

I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...

07 August 2024 505 3 View

How to make a Lateral flow control line more uniform?

We have a lateral flow test on Sartorius 140 NC. The conjugate is gold-monoclonal antibody. Using different control lines (GAM, protein A, protein G) we get a very strong leading edge with reduced...

31 July 2024 4,510 3 View

Why my IgG antibody looks fragmented on non-reducing SDS-PAGE?

I am performing IgG purification and I have to show my results on SDS-PAGE. I use 10% tris glycine gel and prepare the samples under non reducing conditions. I am new to antibodies and therefore...

23 July 2024 6,664 6 View

What are the strategies to Enhance IgG-Producing Plasma Cells in mice for Monoclonal Antibody Development?

I have immunized BalB/C mice with a protein using the intradermal (ID) method with Complete Freund's Adjuvant (CFA) and Incomplete Freund's Adjuvant (IFA), following a 14-day interval and three...

22 July 2024 9,160 2 View

What are those black/hollow structures?

Hello, I recently stained my cells with an Arf6 antibody. The healthy control looks pretty normal, while the disease group cells have abnormally large black structures in the cell body. Has anyone...

21 July 2024 1,044 1 View

How to activate 4E8 T cells with plate-bound anti-CD3 for a genome-wide CRISPR screen?

It was assumed that 4E8 T cells should be isolated and activated using plate-bound anti-human CD3, after which would be used for a genome-wide CRISPR screen. (Ref: Genome-wide CRISPR Screens in...

17 July 2024 3,371 0 View

Questions of antibodies selection?

Hi there, I'd like to use immunocytochemistry to determine a surface protein expression on mouse cell. The primary antibody I have is mouse anti mouse, and the secondary antibody I have is goat...

15 July 2024 6,008 5 View

Trouble finding an anti-Digoxigenin antibody to perform Southern Blot technique?

I'm currently using Roche anti-DIG-AKP Fab fragments to perform Southern Blot assays, but this reagent is delayed since november last year. It's anybody facing the same problem? If so, how are you...

15 July 2024 6,668 0 View

How to measure the concentration of monoclonal antibodieswithout nanodrop ?

Dear all, I'm going to radiolabel antibodies. So first of all, I'll remove excipients using the centrifugation method with amicon. Once I'm done, how do I calculate/measure the concentration of...

14 July 2024 3,106 3 View

Mitali Das

Life Technologies is a pretty reliable source of highly cross-adsorbed, fluorescent tagged antibodies against different species.

Maan Zrein

For cost-effective reagents of this type, check: www.abliance.com

Elena Yu. Filinova

Abcam

Russell Linscott

We'd also suggest Jackson Immunoresearch: https://www.jacksonimmuno.com/

and KPL: http://www.kpl.com