Anyone who knows a good virus for the retrogarde expression of DREADDs in cre-mice ?

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How Download/Extract citations of 1 given year ALL my publications in Google Scholar?

If you google this you find a lot of smart guys explaining you how to download 1 year of citations of 1 single paper of yours (or anyone else). I mean 1 year of ALL my papers. or ... ALL years or...

01 March 2021 1,121 9 View

What keeps journals from switching to a "publish, then review" model of publishing?

A few scientific journals have started implementing a "publish, then review" model of publishing. It is explained well in this article: Implementing a "publish, then review" model of...

13 January 2021 5,449 2 View

Is there any project for a new translation of Freud's 1895 Entwurf einer Psychologie into English?

I'm far from being a Freud expert, but, my feeling is the 1950 translation by J. Strachey is in many ways outdated, when not misleading (starting form the very title of the essay, the awful choice...

10 December 2020 7,005 2 View

What is the main difference of diluting HCL in methanol as opposed to water?

I am hoping to determine the mass per unit area (as per DIN 50938) of a black oxide coating. I use water to achieve the required 1mol/l hydrochloric acidic solution, but a fellow lab had used...

06 December 2020 4,806 1 View

Linear Mixed Models for repeated measures - how can I restructure my data set when some variables are only collected at baseline?

Hello, I plan to perform a linear mixed model analysis to look at change in cognitive function over 5 follow up waves. I am interested in looking at how this change is influenced by dietary and...

27 November 2020 5,087 3 View

Mendeley profiles will be removed in December. What will be of the reader counts from Mendeley as early impact predictors?

Elsevier announced that Mendeley profiles will be discontinued from December. There is a study (Mendeley readership counts: An investigation of temporal and... ) that confirms the value of...

01 November 2020 4,832 4 View

Assessing MCMC convergence by matrix-parameter?

Hello everyone, I need to assess whether a MCMC chain (implemented in BayPass 2.2) has reached convergence and, as far as I know, in order to check it, I must look at the posterior distribution of...

27 October 2020 6,170 3 View

Searching for reliable antibodies for the adrenergic receptors alpha1D and alpha2A for western blotting?

Dear all, I am looking for reliable antibodies for the adrenergic receptors alpha1D and alpha2A, to use in Western Blotting on human skeletal muscle tissue. Anyone with experience with good...

25 October 2020 9,421 2 View

What is the best way to start and develop a scientific collaboration?

What advice would you give to a young researcher who has to develop his/her ability to produce collaborative scientific work?

22 October 2020 5,934 8 View

Temporal noise analysis CMOS APS?

I was reading the paper "Analysis of Temporal Noise in CMOS Photodiode Active Pixel Sensor" but I am a bit puzzled about noise calculation during integration. In particular, can someone explain...

15 October 2020 9,597 3 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View

How to avoid ulcers in s.c. injected tumors ?

Recently, we have injected s.c. B16F10 tumor cells in BL/6 mice. At around 12-15th day some animals showed ulcerations in tumors , with a big hole in the middle (it looks like a volcano). We have...

01 March 2021 9,938 3 View

How vaccines of covid 19 work?

Millions ask how vaccines of covid 19 be successful and safe, its here to explain generally how vaccine of pfizer work ,mRNA vaccines teach our cells how to make a protein that will trigger an...

28 February 2021 2,723 4 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Looking for genomic DNA extraction kits from mouse tissue (centrifuge-based, not spin-column)?

Hello. I am looking to extract genomic DNA from mouse brain and lungs and I need a kit that is not spin-column based because I need to scale up the solution amounts for 400mg tissue (most cap at...

25 February 2021 155 2 View

What are the pros and cons for mice versus rats?

Hi Everyone, I am designing a pilot study using rodents to assess the effects of simulated space environments (radiation + altered gravity) on microbiome and immune system. I have limited...

24 February 2021 1,825 3 View

Is it harmful to breed for Cre Homozygosity initially?

Hello, I am currently using a Cas9 knock in system that will be expressed in the liver. To do so STOP codon fl/fl Cas9 KI mice were bred with Albumin-Cre mice to generate heterozgous pups, that...

24 February 2021 5,759 3 View

How to empty large intestine in mice ?

Hi all I am working on colon-targeted drug delivery for which I kept mice fasting for 6h using fasting grills during scotophase (dark period). I observed liquid fecal content in the cecum and...

23 February 2021 7,701 2 View