Anyone who knows a good virus for the retrogarde expression of DREADDs in cre-mice ?

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Where should the ARS sequence be introduced on a plasmid?

Hi all, I need to introduce an ARS (autonomously replicating sequence) in my plasmid but I'm not sure which position would be the best. Does anyone have any suggestion? A picture of the plasmid...

05 August 2024 1,573 4 View

Use magnetic bead purification after gel electrophoresis?

Hi, I am working with Invitrogen Seamless cloning which requires 200 ng/ul insert DNA fragment. Protocol recommends to use restriction enzyme to digest pre-cloned plasmid and then elute specific...

25 June 2024 971 2 View

How to set up focus groups in the face of generational barriers?

I am carrying out a research project in which I am conducting focus groups with older men and women to study the musical stereotypes learned in traditional Spanish oral music groups, but I am...

10 May 2024 3,852 3 View

What is the best LC/MS system for surfactant analysis?

We are an R&D laboratory focused on anionic, cationic, and zwitterionic surfactants and usually tackle surfactant synthesis and analysis of products from customers. In particular, we deal with...

29 April 2024 8,927 1 View

Why is there no 28S RNA in mouse sceletal muscle?

We are isolating total RNA from mouse sceletal muscle, using the beads beater with the sample already being in Trizol, afterwards chloroform extraction and finally Qiagen RNeasy kit. On the TAPE...

29 April 2024 3,952 5 View

What’s the most used inclusive leadership assessment scale?

I’m looking for an inclusive leadership scale to include in a mediation analysis with psych safety and workplace belonging, what would you use

27 April 2024 6,072 2 View

COMET ASSAY: Why do cells disappear?

Greetings, I am trying to set up an alkaline Comet Assay. I have no previous experience with this type of assay, unfortunately, so I am doing a lot of trial and error. After embedding my cells in...

23 April 2024 6,534 0 View

In what ways does the Sysmex DI-60 streamline laboratory workflow and improve laboratory efficiency in terms of reduced TAT?

This question is directed on the performance of the Sysmex DI-60 digital morphology analyzer and its contributions on the improvement in the laboratory workflow. I am curious as to what...

10 April 2024 3,069 1 View

Aside from a lack of understanding, what other variables contribute to antibiotic diversion in low-income countries such as the Philippines?

It is known that self-medication with antibiotics is an arising problem, becoming a major constituent to antibiotic resistance. Moreover, self-medication heightens the possibility of developing...

09 April 2024 6,644 1 View

What are the most prevalent antibiotic resistance patterns among outpatient populations in the Philippines?

Antibiotic resistance is a pressing global and local health concern. Outpatient settings play a crucial role in antibiotic consumption and the emergence of resistant bacteria due to the frequent...

09 April 2024 6,004 0 View

How can I prepare virus for a TEM or SEM imaging?

I have virus (viral hemorrhagic septicemia virus) in suspension and the experiment will not involve cells. What level of TCID50 is preferred?

11 August 2024 3,115 1 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Mouse CD3 antibody sequence?

Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much

08 August 2024 9,157 4 View

Can I multiplex a mouse monoclonal and rat primary for IHC (mouse brain tissue)?

Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...

06 August 2024 9,710 1 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

In what part of the brain´s rabbit bdnf is located and include references please?

I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....

04 August 2024 762 1 View

Tissue homogenization in mouse with FastPrep-24™ 5G - is the D matrix system useful?

I need to lyse and homogenise mouse tissues. So far I have experimented with liver and lung tissue, using matrix D. The liver was completely lysed with 6 repetitions of the standard program, but...

31 July 2024 9,186 0 View

How to Post-fix Mice pup's brains?

I need to Postfix mice brains. I slice the brains using microtome at 40 microns. These are Postnatal days 7, 11, 14, and 21. I am using half the brain for other analysis, so I need to fresh freeze...

31 July 2024 1,123 4 View

How to prevent hemolysis after performing cardiac puncture to draw blood from mice in serum?

We have encountered an issue with the serum of mice being hemolyzed after the process of centrifugation. Could you please provide guidance to prevent hemolysis ?

29 July 2024 8,402 4 View