I have a DNA sample and I don't know if it is good or bad, I want to perform PCR.
I want to be sure that the bands that will appear after PCR are the desired amplicon and not a primer-dimer. I need a positive and negative control for my PCR reaction but I don't know how to set up the controls.
Your help will be highly appreciated.
Avinash Sharma
As described by Dr. Ali.... Use any amplified DNA from your study as a positive control and don't add DNA in the negative control. Make sure the DNA you are using for the positive control is already used to amplify the similar kind of gene.
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